| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
I examined whether associative learning could be performed at the level of single isolated Kenyon cell from the cricket mushroom bodies. Pressure ejection of acetylcholine (ACh) and octopamine (OA) via drug-filled pipettes was used as a conditioned stimulus (CS) and an unconditioned stimulus (US), respectively. As a possible target molecule during CS-US paring conditioning, Na+-activated K+ (KNa) channels were used. The present results showed that 5 trial CS-US paired conditioning caused an alteration of responsibility of KNa channels to ACh. This alteration was not observed when backward paring (US-CS) of the conditioning was used.This alteration was also not observed in the presence of NOS inhibitor,IP3 receptor inhibitor, PKG inhibitor, and PKA inhibitor in the bath solution. These results indicate that the cross-talk between NO/cGMP/PKG signaling and cAMP/PKA signaling may play an important role in the alteration of the responsibility of KNa channels to ACh.
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