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Molecular mechanisms underlying the classical conditioning with an isolated Kenyon cell

Research Project

Project/Area Number 15K07145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionTokyo Gakugei University

Principal Investigator

YOSHINO Masami  東京学芸大学, 教育学部, 名誉教授 (20175681)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsclassical conditioning / Kenyon cell / ion channel / acetylcholine / octopamine / 古典的条件付け / ケニオン細胞 / イオンチャネル / アセチルコリン / オクトパミン / フタホシコオロギ / イオンチャンル / パッチクランプ / 条件刺激 / 無条件刺激
Outline of Final Research Achievements

I examined whether associative learning could be performed at the level of single isolated Kenyon cell from the cricket mushroom bodies. Pressure ejection of acetylcholine (ACh) and octopamine (OA) via drug-filled pipettes was used as a conditioned stimulus (CS) and an unconditioned stimulus (US), respectively. As a possible target molecule during CS-US paring conditioning, Na+-activated K+ (KNa) channels were used. The present results showed that 5 trial CS-US paired conditioning caused an alteration of responsibility of KNa channels to ACh. This alteration was not observed when backward paring (US-CS) of the conditioning was used.This alteration was also not observed in the presence of NOS inhibitor,IP3 receptor inhibitor, PKG inhibitor, and PKA inhibitor in the bath solution. These results indicate that the cross-talk between NO/cGMP/PKG signaling and cAMP/PKA signaling may play an important role in the alteration of the responsibility of KNa channels to ACh.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Nitric oxide augments single persistent Na+ channel currents via the cGMP/PKG signaling pathway in Kenyon cells isolated from the cricket mushroom bodies2018

    • Author(s)
      Mariko Ikeda and Masami Yoshino
    • Journal Title

      Journal of Neurophysiology

      Volume: in press Issue: 2 Pages: 720-728

    • DOI

      10.1152/jn.00440.2017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitric oxide/cGMP/PKG signaling pathway activated by M1-type muscarinic acetylcholine receptor cascde inhibits Na-activated K currents in Kenyon cells2016

    • Author(s)
      Hasebe M, Yoshino M
    • Journal Title

      Journal of Neurophysiology

      Volume: 115 Issue: 6 Pages: 3174-3185

    • DOI

      10.1152/jn.00036.2015

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nitri oxide augments single Ca channel currents via cGMP-dependent protein kinase in Kenyon cells isolated from the mushroom body of the cricket brain.2015

    • Author(s)
      Kumiko Kosakai, Yuuki Tsujiuchi, Masami Yoshino
    • Journal Title

      Journal of Insect Physiology

      Volume: 78 Pages: 26-32

    • DOI

      10.1016/j.jinsphys.2015.04.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional coupling between sodium-activated potassium channels and voltage-dependent persistent sodium currents in cricket Kenyon cells.2015

    • Author(s)
      Takahashi I, Yoshino M
    • Journal Title

      Journal of Nurophysiology

      Volume: 114 Issue: 4 Pages: 2450-2459

    • DOI

      10.1152/jn.00087.2015

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ケニオン細胞における内因性・外因性NOによる持続性Na電流の調節2017

    • Author(s)
      池田真理子、吉野正巳
    • Organizer
      第94回日本生理学会大会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] 単一ニューロンにおける連合学習2017

    • Author(s)
      高橋 泉、吉野正巳
    • Organizer
      第94回日本生理学会大会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] Modulation of native ionic channels by nitric oxide and acetylcholine signaling pathways in Kenyon cell s isolated from the mushroom body of the cricket brain.2016

    • Author(s)
      Masami Yoshino
    • Organizer
      2016 International Congress of Entomology
    • Place of Presentation
      Orlando, Florida
    • Year and Date
      2016-09-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Modulation of single sodium channel currents by nitric oxide signaling pathway in insect memory center neurons isolated from the mushroom body of the cricket brain.2016

    • Author(s)
      Mariko Ikeda, Masami Yoshino
    • Organizer
      The Journal of Physiological Sciences, Proceedings of the 93rd Annual Meeting
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Functional coupling between sodium-activated potassium channels and voltage-dependent persistent sodium currents in cricket Kenyon cells2016

    • Author(s)
      Izumi Takahashi, Masami Yoshino
    • Organizer
      The Journal of Physiological Sciences, Proceedings of the 93rd Annual Meeting
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Neuromodulatory action of nitric oxide on memory center neurons called Kenyon cells in insect2016

    • Author(s)
      Masami Yoshino
    • Organizer
      The Journal of Physiological Sciences, Proceedings of the 93rd Annual Meeting
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] 昆虫脳ニューロンの膜興奮性とイオンチャネル2016

    • Author(s)
      吉野正巳
    • Organizer
      日本動物学会第68回関東支部大会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2016-03-12
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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