Visual neural activity regulates functional restoration of the photorecognition via neuropeptides during planarian brain regeneration

• Project/Area Number: 15K07148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Gakushuin University (2016-2017); Kyoto University (2015)
• Principal Investigator: Inoue Takeshi 学習院大学, 理学部, 助教 (40391867)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 活動依存的 / 神経ペプチド / 脳再生 / 機能回復 / 環境応答行動 / 光応答行動 / プラナリア / 螺旋動物 / 環境適応行動 / 活動依存的発達 / RNAi / 環境応答 / 神経科学 / 行動

Outline of Final Research Achievements

A variety of external signals interpreted into defined neural functions play important role in the refinement of the neural function. Much remains to be understood how external signals contribute to the formation of the efficient neural system that supporting the transformation of the appropriate behavioral response against the complicated stimulus. Planarians display behaviors responsive to signals coming from outside such as light avoidance behavior, and the nervous system can be regenerated and its function completely restored even after decapitated, since the extraordinary regenerative capacity of planarians. We analyzed the molecular mechanisms of behavioral recovery during brain regeneration using RNAi and a behavioral assay in the planarian. Here we show that the novel neuropeptides contribute to the restitution of the proper phototactic behavior by modulating the photo-recognition during brain regeneration.

Research Products

• [Journal Article] Functional Specification of a Primitive Bilaterian Brain in Planarians (2017)
  • Author(s): Inoue Takeshi
  • Journal Title: Brain Evolution by Design Volume: 1 Pages: 79-100
  • DOI: 10.1007/978-4-431-56469-0_4
  • ISBN: 9784431564676, 9784431564690
  • Peer Reviewed
• [Journal Article] Multiple neuropeptide-coding genes involved in planarian pharynx extension (2016)
  • Author(s): Shimoyama, S., Inoue, T., Kashima, M. and Agata, K.
  • Journal Title: Zool Sci Volume: 印刷中
  • NAID: 210000165369
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Control of maintenance and regeneration of planarian eyes by ovo (2015)
  • Author(s): Cross, S. D., Johnson, A. A., Gilles, B. J., Bachman, L. A., Inoue, T., Agata, K., Marmorstein, L. Y. and Marmorstein, A. D.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 56 Issue: 12 Pages: 7604-7610
  • DOI: 10.1167/iovs.15-17458
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Unusually large number of mutations in asexually reproducing clonal planarian Dugesia japonica (2015)
  • Author(s): Nishimura, O., Hosoda, K., Kawaguchi, E., Yazawa, S., Hayashi, T., Inoue, T., Umesono, Y. and Agata, K.
  • Journal Title: PLoS One Volume: 10 Issue: 11 Pages: e0143525-e0143525
  • DOI: 10.1371/journal.pone.0143525
  • NAID: 120005749906
  • Peer Reviewed / Open Access
• [Journal Article] Spontaneous behaviors and wall-curvature lead to apparent wall preference in planarian (2015)
  • Author(s): Akiyama, Y., Agata, K. and Inoue, T.
  • Journal Title: PLoS One Volume: 10 Issue: 11 Pages: e0142214-e0142214
  • DOI: 10.1371/journal.pone.0142214
  • NAID: 120005743646
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Close relationship between the eye architecture and the spontaneous self-motion establishes robust photo-response orientation behavior in planarians (2018)
  • Author(s): Inoue, T.
  • Organizer: The International Research Symposium on Germness and Pluripotency of the Planarians in comparison with the Fly and Mouse Systems
  • Int'l Joint Research / Invited
• [Presentation] Binocular field and spontaneous self-motion specify efficient phototactic behavior in planarians (2017)
  • Author(s): 秋山義太郎、阿形清和、●井上武
  • Organizer: 日本比較生理生化学会第39会大会
• [Presentation] プラナリアは自発的運動によって偽壁嗜好性行動を示す (2017)
  • Author(s): 秋山義太郎、阿形清和、●井上武
  • Organizer: 日本動物学会第88回大会
• [Presentation] プラナリアでは両眼視野と自発的運動が走光性行動の効率を決定する (2017)
  • Author(s): 秋山義太郎、阿形清和、●井上武
  • Organizer: 第3回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
• [Presentation] External signals restore the brain function via novel neuropeptide up-regulation during brain regeneration (2015)
  • Author(s): T.Inoue, T. Takano, Y. Umesono, and K. Agata
  • Organizer: 第 40 回日本比較内分泌学会大会 日本比較生理生化学会第37回大会 合同大会
  • Place of Presentation: 広島市、JMS アステールプラザ
  • Year and Date: 2015-12-11
• [Presentation] RNAi を用いたプラナリアの摂食行動に関わる神経系の同定 (2015)
  • Author(s): 下山せいら、井上武、阿形清和
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟市、朱鷺メッセ
  • Year and Date: 2015-09-17
• [Presentation] プラナリアにおける温度走性を制御する脳機能 (2015)
  • Author(s): 井上武
  • Organizer: 日本動物学会第86回大会 第5回ホメオスタシスバイオロジーシンポジウム「温度受容と環境適応のメカニズム」
  • Place of Presentation: 新潟市、朱鷺メッセ
  • Year and Date: 2015-09-17
  • Invited
• [Presentation] Planarian feeding behavior (2015)
  • Author(s): S. Shimoyama, K. Agata, T. Inoue
  • Organizer: 4th International Seminar on Biodiversity and Evolution
  • Place of Presentation: Kyoto University, Kyoto
  • Year and Date: 2015-06-09
  • Int'l Joint Research
• [Book] 遺伝子から解き明かす脳の不思議な世界 第3章 扁形動物と脳の再生 (2018)
  • Author(s): 井上 武
  • Total Pages: 20
  • Publisher: 一色出版
• [Remarks] planarian genome database