| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Recently, it has been reported that D-amino acids were found in many animals and have various physiological functions. Additionally, D-amino acids are known to be synthesized by the amino acid racemase. In this study, we isolated the amino acid racemases by novel screening method using the amino acid auxotrophic E. coli. Furthermore, we searched homologues of amino acid racemase from GenBank DNA sequence databases. Subsequently, the enzyme activity of these amino acid racemase genes was confirmed using a recombinant enzymes. This study revealed that serine racemase and aspartate racemase are widely distributed in the animal kingdom.
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