| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
I demonstrated evidence that a SUMO-interacting motif (SIM) resided on lamin A polypeptide is required for dephosphorylation of lamin A during telophase and subsequent nuclear lamina formation. I tried to identify factors which allow the recruitment of lamin A to the telophase chromosome and dephosphorylation of the mitotic lamin A phosphorylation. As a result, I found that RepoMan, a regulatory subunit of protein phosphatase 1gamma contributes to lamin A recruitment to telophase chromosomes and dephosphorylation of the mitotic lamin A phosphorylation. Expression of a SUMO2 mutant defective in SUMO-SIM interaction resulted in failure of the lamin A and RepoMan association, along with abrogation of lamin A dephosphorylation and subsequent nuclear lamina formation. These findings strongly suggested that RepoMan/PP1gamma recruits lamin A through SUMO-SIM interaction.
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