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Identification of factors interacting with lamin A on the telophase chromosome

Research Project

Project/Area Number 15K07161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genetics/Chromosome dynamics
Research InstitutionUniversity of Hyogo

Principal Investigator

Hirose Fumiko  兵庫県立大学, 生命理学研究科, 准教授 (60208882)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsSUMO化 / 核ラミナ / 有糸分裂期 / 脱リン酸化 / クロマチン / ヘテロクロマチン / 分裂期 / 核膜 / ラミンA / 翻訳後修飾 / 分裂期終期
Outline of Final Research Achievements

I demonstrated evidence that a SUMO-interacting motif (SIM) resided on lamin A polypeptide is required for dephosphorylation of lamin A during telophase and subsequent nuclear lamina formation. I tried to identify factors which allow the recruitment of lamin A to the telophase chromosome and dephosphorylation of the mitotic lamin A phosphorylation. As a result, I found that RepoMan, a regulatory subunit of protein phosphatase 1gamma contributes to lamin A recruitment to telophase chromosomes and dephosphorylation of the mitotic lamin A phosphorylation. Expression of a SUMO2 mutant defective in SUMO-SIM interaction resulted in failure of the lamin A and RepoMan association, along with abrogation of lamin A dephosphorylation and subsequent nuclear lamina formation. These findings strongly suggested that RepoMan/PP1gamma recruits lamin A through SUMO-SIM interaction.

Academic Significance and Societal Importance of the Research Achievements

遺伝物質であるDNAが細胞分裂を経た後も正確に2つの娘細胞に受け継がれる。細胞分裂では染色体の分配と分離を正確に遂行するための仕組みがあり、リン酸化と脱リン酸化のカスケードを介した緻密な制御機構が明らかとなっている。一方で、分裂期の最後に形成される核ラミナと核膜形成のタイミングや場所を調節する仕組みについては不明な点が多かった。本研究では、核ラミナと核膜を再形成させるきっかけが、核ラミナの構成因子であるラミンAたんぱく質の脱リン酸化であり、これを行う酵素が分裂期終期の染色体上にあることを明らかにした。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) Remarks (4 results)

  • [Journal Article] Transcription factor hDREF is a novel SUMO E3 ligase of Mi2alpha2017

    • Author(s)
      Yamashita D, Moriuchi T, Osumi T and Hirose F.
    • Journal Title

      J. Biol. Chem.

      Volume: 291 Issue: 22 Pages: 11619-11634

    • DOI

      10.1074/jbc.m115.713370

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Lamin A reassembly at the end of mitosis is regulated by its SUMO-interacting motif.2015

    • Author(s)
      Moriuchi T., Kuroda, M., Kusumoto F., Osumi, T. and Hirose F.
    • Journal Title

      Exp. Cell Res.

      Volume: 342 Issue: 1 Pages: 83-94

    • DOI

      10.1016/j.yexcr.2016.02.016

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Assembly of heterochromatin under the nuclear membrane is determined at the end of mitosis2018

    • Author(s)
      河合淳史、廣瀬富美子
    • Organizer
      日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dephosphorylation of lamin A at the end of mitosis is regulated by SUMOylation2017

    • Author(s)
      廣瀬富美子
    • Organizer
      分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] RepoMan/PP1γ複合体は分裂期終期におけるlamin Aの脱リン酸化に関わる2016

    • Author(s)
      森内昂文、長栄良平、木藤健太、廣瀬富美子
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] SUMO-SIM相互作用による分裂期終期の核膜構築の制御2016

    • Author(s)
      有糸分裂期におけるRepoManのSUMO化の解析
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] SUMO-SIM相互作用による分裂期終期の核膜構築の制御2016

    • Author(s)
      廣瀬富美子
    • Organizer
      第13回SUMO研究会
    • Place of Presentation
      関西学院大学大阪梅田キャンパス(大阪府大阪市)
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
  • [Presentation] 分裂期におけるラミンAの核ラミナ再構築に必要な領域の決定2015

    • Author(s)
      楠本 史也、森内 昂文、廣瀬 富美子、大隅 隆
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 分裂期終了時の核ラミナ再構築はSUMO化によって制御される2015

    • Author(s)
      森内 昂文、大隅 隆、廣瀬 富美子
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] M期におけるラミンA- RepoMan間相互作用の時空間的解析2015

    • Author(s)
      長栄 良平、森内 昂文、木藤 健太、大隅 隆、廣瀬 富美子
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Remarks] 兵庫県立大学大学院 生命理学研究科細胞機能学講座HP

    • URL

      http://u-hyogo-cellandmolecularbiology.strikingly.com/#home

    • Related Report
      2018 Annual Research Report
  • [Remarks]

    • URL

      http://u-hyogo-cellandmolecularbiology.strikingly.com/

    • Related Report
      2017 Research-status Report
  • [Remarks] 兵庫県立大学大学院 生命理学研究科細胞機能学講座

    • URL

      http://u-hyogo-cellandmolecularbiology.strikingly.com/

    • Related Report
      2016 Research-status Report
  • [Remarks] 兵庫県立大学大学院細胞機能学分野

    • URL

      http://u-hyogo-cellandmolecularbiology.strikingly.com/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

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