| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB and NF-YC. It suggests that dNF-YA genetically interact with lipase4(lip4) gene by genetic cross. The 5’ franking region of lip4 gene has Three NF-Y binding consensus sequences (YYRRCCAATCAG) which play negative roles in promoter activity. In chromatin immunoprecipitation assays with anti-dNF-YA antibody, the lip4 gene 5’-flanking region containing the NF-Y consensus was effectively amplified in immunoprecipitates by real time qPCR. These results suggest that dNF-Y involved in metabolism of lipid by transcriptional regulation of lip4 gene.
The specific knockdown of dNF-YA in fat body resulted that reduction of oil drop on fatbody. These results suggest that dNF-YA involved in fat metabolism in vivo. These observations combined with other cytological, genetical and molecular biological studies indicate that dNF-Y regulates Lip4 gene expression during metabolism of fat on Drosophila.
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