Analysis of regulation of lipid metabolism by transcription factor NF-Y on Drosophila fat body
| Project/Area Number |
15K07162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Setsunan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山口 政光 京都工芸繊維大学, 応用生物学系, 教授 (00182460)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 脂肪体 / NF-Y / 脂質代謝 / ショウジョウバエ / Lipase遺伝子 / 転写 |
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| Outline of Final Research Achievements |
The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB and NF-YC. It suggests that dNF-YA genetically interact with lipase4(lip4) gene by genetic cross. The 5’ franking region of lip4 gene has Three NF-Y binding consensus sequences (YYRRCCAATCAG) which play negative roles in promoter activity. In chromatin immunoprecipitation assays with anti-dNF-YA antibody, the lip4 gene 5’-flanking region containing the NF-Y consensus was effectively amplified in immunoprecipitates by real time qPCR. These results suggest that dNF-Y involved in metabolism of lipid by transcriptional regulation of lip4 gene.
The specific knockdown of dNF-YA in fat body resulted that reduction of oil drop on fatbody. These results suggest that dNF-YA involved in fat metabolism in vivo. These observations combined with other cytological, genetical and molecular biological studies indicate that dNF-Y regulates Lip4 gene expression during metabolism of fat on Drosophila.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] NF-Y in invertebrates.2016
Author(s)
Yamaguchi M, Ali MS, Yoshioka Y, Ly LL, Yoshida H.
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Journal Title
Biochim Biophys Acta.
Volume: 16
Issue: 5
Pages: 630-635
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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