| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Mouse embryonic stem (ES) cells contain a small population expressing zygotic genes usually transcribed at 2-cell stage (2CG). Base on the previous finding, 2CG-high cells induced by Rif1 knockdown were precisely analyzed in this study. The main results were as follows; 1. mRif1 knockdown lead to massive derepression of 2CG and endoretrovirus Merv-L. 2. DNA methylation at Zscan4 loci was not substantially reduced. 3. H3K27Ac was noticeably increased in Zscan4 gene loci and formed enhancer-like structure for at least 20 kb. Futheremore, Transient expression of mRif1 deletion mutant without N-terminal HEAT repeat structure could induce 2CG-high states.
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