Functional analysis of Rif1 in regulating muticopy gene cluster and super state of pluripotent stem cells
| Project/Area Number |
15K07164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
YOSHIZAWA Naoko 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (30344071)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Rif1 / 初期胚 / 2細胞期 / 内在性レトロウィルス / エンハンサー / Zscan4 / ヒストン修飾 / DNAメチル化 / H3K27Ac / 遺伝子クラスター / ２細胞期 / トランスポゾン / ２細胞期初期胚 |
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| Outline of Final Research Achievements |
Mouse embryonic stem (ES) cells contain a small population expressing zygotic genes usually transcribed at 2-cell stage (2CG). Base on the previous finding, 2CG-high cells induced by Rif1 knockdown were precisely analyzed in this study. The main results were as follows; 1. mRif1 knockdown lead to massive derepression of 2CG and endoretrovirus Merv-L. 2. DNA methylation at Zscan4 loci was not substantially reduced. 3. H3K27Ac was noticeably increased in Zscan4 gene loci and formed enhancer-like structure for at least 20 kb. Futheremore, Transient expression of mRif1 deletion mutant without N-terminal HEAT repeat structure could induce 2CG-high states.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Telomere-binding factors in the regulation of DNA replication2017
Author(s)
Masai, H., Kanoh, Y., Moriyama, K., Yamazaki, S., Yoshizawa, N., and Matsumoto, S.
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Journal Title
Genes & Genetic Systems
Volume: 92
Issue: 3
Pages: 119-125
DOI
NAID
ISSN
1341-7568, 1880-5779
Related Report
Peer Reviewed / Open Access
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