| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We identified Cmb1 as a mitochondrial DNA-binding protein in the fission yeast, S. pombe. In order to reveal the evolution of the mitochondrial DNA-binding protein, we compared the function of Cmb1 to that of Abf2p, a major mitochondrial DNA-binding protein of the budding yeast, S. cerevisiae. We examined whether Cmb1 at the different expression levels can complement the instability of mitochondrial DNA in the abf2-deficient cells of S. cerevisiae. As a result, we demonstrated that Cmb1 and Abf2p show different complementation against the abnormal morphology of mitochondrial nucleoids, loss of respiratory activity in high temperature, and high sensitivity against ethidium bromide of abf2-deficient cells. These results indicate that the function of the mitochondrial DNA-proteins diverged during evolution of the yeasts.
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