Illumination of early stages of mitochondrial adaptation to low oxygen

• Project/Area Number: 15K07176
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Fukuoka Women's University (2017); Japan Agency for Marine-Earth Science and Technology (2015-2016)
• Principal Investigator: Takishita Kiyotaka 福岡女子大学, 国際文理学部, 教授 (90392951)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 原生生物 / 嫌気 / ミトコンドリア

Outline of Final Research Achievements

The biochemical characteristics of an mitochondrion-related organelles (MROs) in the free-living anaerobic protist Cantina marsupialis, which represents an independent lineage in stramenopiles, were inferred based on RNA-seq data. We found transcripts for proteins known to function in one form of MROs, the hydrogenosome. In addition, MROs in C. marsupialis were also shown to share several features with canonical mitochondria, including amino acid metabolism and an "incomplete" tricarboxylic acid cycle. Transcripts for all four subunits of complex II (CII) of the electron transport chain were detected, while there was no evidence for the presence of complexes I, III, IV, or F1Fo ATPase. CII in MROs of C. marsupialis may function as succinate dehydrogenase rather than as fumarate reductase.

Research Products

• [Journal Article] Comparison of morphological and DNA-based techniques for stomach content analyses in juvenile chum salmon Oncorhynchus keta: a case study on diet richness of juvenile fishes (2017)
  • Author(s): Sakiko Orui Sakaguchi, Shigeru Shimamura, Yuichi Shimizu, Gen Ogawa, Yuichiro Yamada, Keiko Shimizu, Hiroaki Kasai, Hiroshi Kitazato, Yoshihiro Fujiwara, Katsunori Fujikura, Kiyotaka Takishita
  • Journal Title: Fisheries Science Volume: 83 Issue: 1 Pages: 47-56
  • DOI: 10.1007/s12562-016-1040-6
  • NAID: 40021063202
  • Peer Reviewed
• [Journal Article] Updated mitochondrial phylogeny of Pteriomorph and Heterodont Bivalvia, including deep-sea chemosymbiotic Bathymodiolus mussels, vesicomyid clams and the thyasirid clam Conchocele cf. bisecta (2017)
  • Author(s): Ozawa, G., S. Shimamura, Y. Takaki, S. Yokobori, Y. Ohara, K. Takishita, T. Maruyama, K. Fujikura and T. Yoshida
  • Journal Title: Marine Genomics Volume: 31 Pages: 43-52
  • DOI: 10.1016/j.margen.2016.09.003
  • Peer Reviewed
• [Journal Article] Organelles that illuminate the origins of Trichomonas hydrogenosomes and Giardia mitosomes (2017)
  • Author(s): Leger M, Kolisko M, Ryoma Kamikawa, Stairs CW, Kume K, Cepicka I, Silberman JD, Andersson JO, Xu F, Yabuki A, Zhang Q, Takishita K, Inagaki Y, Simpson AGB, Hashimoto T, Roger AJ
  • Journal Title: Nature Ecology and Evolution Volume: 1 Issue: 4 Pages: 0092-0092
  • DOI: 10.1038/s41559-017-0092
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genomic evidence that methanotrophic endosymbionts likely provide deep-sea Bathymodiolus mussels with a sterol intermediate in cholesterol biosynthesis (2017)
  • Author(s): Kiyotaka Takishita, Yoshihiro Takaki, Yoshito Chikaraishi, Tetsuro Ikuta, Genki Ozawa, Takao Yoshida, Naohiko Ohkouchi, Katsunori Fujikura
  • Journal Title: Genome Biology and Evolution Volume: 9 Issue: 5 Pages: 1148-1160
  • DOI: 10.1093/gbe/evx082
  • Peer Reviewed
• [Journal Article] Cladomarine, a new anti-saprolegniasis compound isolated from the deep-sea fungus, Penicillium coralligerum YK-247 (2017)
  • Author(s): Takahashi Konami、Sakai Kazunari、Nagano Yuriko、Orui Sakaguchi Sakiko、Lima Andre O、Pellizari Vivian H、Iwatsuki Masato、Takishita Kiyotaka、Nonaka Kenichi、Fujikura Katsunori、?mura Satoshi
  • Journal Title: The Journal of Antibiotics Volume: 70 Issue: 8 Pages: 911-914
  • DOI: 10.1038/ja.2017.58
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ancient occasional host switching of maternally transmitted bacterial symbionts of chemosynthetic vesicomyid clams (2017)
  • Author(s): Genki Ozawa, Shigeru Shimamura, Yoshihiro Takaki, Kiyotaka Takishita, Tetsuro Ikuta, James P Barry, Tadashi Maruyama, Katsunori Fujikura, Takao Yoshida
  • Journal Title: Genome Biology and Evolution Volume: 9 Issue: 9 Pages: 2226-2236
  • DOI: 10.1093/gbe/evx166
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphological identity of a taxonomically unassigned cytochrome c oxidase subunit I sequence from stomach contents of juvenile chum salmon determined using polymerase chain reaction (2017)
  • Author(s): Sakiko Orui Sakaguchi, Tetsuro Ikuta, Gen Ogawa, Kodai Yamane, Naonobu Shiga, Hiroshi Kitazato, Katsunori Fujikura, Kiyotaka Takishita
  • Journal Title: Fisheries Science Volume: 83 Issue: 5 Pages: 757-765
  • DOI: 10.1007/s12562-017-1106-0
  • NAID: 40021337945
  • Peer Reviewed
• [Journal Article] Microbial Eukaryotes that Lack Sterols (2017)
  • Author(s): Takishita K, Chikaraishi Y, Tanifuji G, Ohkouchi N, Hashimoto T, Fujikura K, Roger AJ
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 64 Issue: 6 Pages: 897-900
  • DOI: 10.1111/jeu.12426
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Complex evolution of two types of cardiolipin synthase in the eukaryotic lineage stramenopiles (2016)
  • Author(s): Noguchi, F., G. Tanifuji, M. W. Brown, K. Fujikura and K. Takishita
  • Journal Title: Molecular Phylogenetics and Evolution Volume: 101 Pages: 133-141
  • DOI: 10.1016/j.ympev.2016.05.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Hyper-eccentric structural genes in the mitochondrial genome of the algal parasite Hemistasia phaeocysticola (2016)
  • Author(s): Yabuki, A., G. Tanifuji, C. Kusaka, K. Takishita and K. Fujikura
  • Journal Title: Genome Biology and Evolution Volume: - Pages: 2870-2878
  • DOI: 10.1093/gbe/evw207
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic capacity of mitochondrion-related organelles in the free-living anaerobic stramenopile Cantina marsupialis (2015)
  • Author(s): Fumiya Noguchi, Shigeru Shimamura, Takuro Nakayama, Euki Yazaki, Akinori Yabuki, Tetsuo Hashimoto, Yuji Inagaki, Katsunori Fujikura, Kiyotaka Takishita
  • Journal Title: Protist Volume: 166 Issue: 5 Pages: 534-550
  • DOI: 10.1016/j.protis.2015.08.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular evidence for wide vertical distribution of the marine planktonic protist Larcopyle buetschlii (Radiolaria) in a semi-closed marginal sea (2015)
  • Author(s): Yoshiyuki Ishitani, Kiyotaka Takishita
  • Journal Title: Journal of Plankton Research Volume: 37 Issue: 5 Pages: 851-856
  • DOI: 10.1093/plankt/fbv065
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Morphological identities of two different marine stramenopile environmental sequence clades: Bicosoeca kenaiensis (Hilliard 1971) and Cantina marsupialis (Larsen and Patterson, 1990) gen. nov., comb. nov. (2015)
  • Author(s): Naoji Yubuki, Tomas Panek, Akinori Yabuki, Ivan Cepicka, Kiyotaka Takishita, Yuji Inagaki, Brian S. Leander
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 62 Issue: 4 Pages: 532-542
  • DOI: 10.1111/jeu.12207
  • Peer Reviewed
• [Presentation] 深海由来真菌の分子同定および抗生物質の探索 (2016)
  • Author(s): 高橋こなみ, 長野由梨子, 瀧下清貴, 藤倉克則, 横田賢史
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター，北海道札幌市
  • Year and Date: 2016-03-29