Investigation on the evolution of the multicellular body plan in plants by focusing on comparative and functional genomics in charophycean algae

• Project/Area Number: 15K07185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Kobe University
• Principal Investigator: Sakayama Hidetoshi 神戸大学, 理学研究科, 准教授 (60391108)
• Co-Investigator(Renkei-kenkyūsha): YAMADA Toshihiro 金沢大学, 自然システム学系, 准教授 (70392537) ; ISHIZAKI Kimitsune 神戸大学, 大学院理学研究科, 准教授 (00452293)
• Research Collaborator: NISHIYAMA Tomoaki ; SUZUKI Yutaka ; KAWAI Hiroshi ; MIMURA Tetsuro ; WATANABE Miyuki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 進化 / 比較ゲノム / シャジクモ藻類 / ストレプト植物 / 多細胞体制 / 植物

Outline of Final Research Achievements

In order to investigate the evolution of the multicellular body plan in land plants and charophycean algae, in this study, we compared their multicellular gametophyte and sporophyte generations based on their genes and genomes. We identified the developmental genes specifically expressed in different tissues/cells of C. braunii. Moreover, we explored the expression/function of these developmental genes in charalean algae in detail and discussed the evolution of the multicellular body plan in land plants and charophycean algae. We were also tried to introduce the exogenous gene into Chara cells using the native promoter of the Chara gene by microinjection and particle bombardment.

Research Products

• [Int'l Joint Research] University of Marburg(ドイツ)
• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] University of Oxford(英国)
• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] University of Wollongong(オーストラリア)
• [Int'l Joint Research] University of Marburg(ドイツ)
• [Int'l Joint Research] University at Buffalo(米国)
• [Int'l Joint Research] University of Oxford(英国)
• [Int'l Joint Research] University of Marburg(ドイツ)
• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research]
• [Journal Article] Taxonomical analysis of closely related species of Chara L. section Hartmania (Streptophyta: Charales) based on morphological and molecular data (2017)
  • Author(s): Urbaniak, J. and Sakayama, H.
  • Journal Title: Fottea Volume: 17 Issue: 2 Pages: 222-239
  • DOI: 10.5507/fot.2017.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] シャジクモ類の和名について (2017)
  • Author(s): 坂山英俊
  • Journal Title: 海洋と生物 Volume: 230 Pages: 229-234
• [Journal Article] Loss of heterophylly in aquatic plants: not ABA-mediated stress but exogenous ABA treatment induces stomatal leaves in Potamogeton perfoliatus (2016)
  • Author(s): Iida, S., Ikeda, M., Amano, M., Sakayama, H., Kadono, Y. and Kosuge, K.
  • Journal Title: Journal of Plant Research Volume: 129 Issue: 5 Pages: 853-862
  • DOI: 10.1007/s10265-016-0844-x
  • Peer Reviewed
• [Journal Article] Diversification of a transcription factor family led to the evolution of antagonistically acting genetic regulators of root hair growth (2016)
  • Author(s): Breuninger, H., Thamm, A., Streubel, S., Sakayama, H., Nishiyama, T. and Dolan, L.
  • Journal Title: Current Biology Volume: 26 Issue: 12 Pages: 1622-1628
  • DOI: 10.1016/j.cub.2016.04.060
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Next-generation sequencing of an 88-year-old specimen of the poorly known species Liagora japonica (Nemaliales, Rhodophyta) supports the recognition of Otohimella gen. nov. (2016)
  • Author(s): Suzuki, M., Segawa, T. Mori, H., Akiyoshi, A., Ootsuki, R., Kurihara, A., Sakayama, H., Kitayama, T., Abe, T., Kogame, K., Kawai, H., Nozaki, H.
  • Journal Title: PLOS ONE Volume: 11 Issue: 7 Pages: e0158944-e0158944
  • DOI: 10.1371/journal.pone.0158944
  • NAID: 120005844143
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system. (2016)
  • Author(s): Kasahara, M., Suetsugu, N., Urano, Y., Yamamoto, C., Ohmori, M., Takada, Y., Okuda, S., Nishiyama, T., Sakayama, H., Kohchi, T., Takahashi, F.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 39232-39232
  • DOI: 10.1038/srep39232
  • Peer Reviewed / Open Access
• [Journal Article] Reconstruction of the former charophyte community out of the oospores identified in Lake Shinji sediments (2016)
  • Author(s): Komuro, T., Sakayama, H., Kamiya, H. and Yamamuro, M.
  • Journal Title: Knowledge and management of aquatic ecosystems Volume: 417 Issue: 417 Pages: 12-12
  • DOI: 10.1051/kmae/2015045
  • Peer Reviewed / Open Access
• [Journal Article] Taxonomy, morphology and genetic variation of Nitella flexilis var. bifurcata (Charales, Characeae) from Japan (2015)
  • Author(s): Sakayama, H., Kai, A, Nishiyama, M., Watanabe, M. M., Kato, S., Ito, M., Nozaki, H. and Kawai, H.
  • Journal Title: Phycological Research Volume: 63 Issue: 3 Pages: 159-166
  • DOI: 10.1111/pre.12085
  • Peer Reviewed
• [Presentation] シャジクモ藻類シャジクモにおけるLEAFY遺伝子ホモログの発現・機能解析 (2018)
  • Author(s): 渡邊みゆき・山田敏弘・西山智明・川井浩史・伊藤元己・坂山英俊
  • Organizer: 日本藻類学会第42回大会
• [Presentation] 西表島の淡水湿地林における着生植物オオタニワタリの空間分布 (2018)
  • Author(s): 吉田智弘・内貴章世・佐藤拓哉・坂山英俊
  • Organizer: 第65回日本生態学会大会
• [Presentation] 培養株を用いたシャジクモの種内系統と生態型の遺伝的基盤に関する研究 (2018)
  • Author(s): 坂山英俊・宮田大輔・加藤 将・川井浩史・西山智明
  • Organizer: 日本植物分類学会第17回大会
• [Presentation] 分離集団の薄いショットガンシーケンシングによるシャジクモ遺伝学的地図の構築 (2018)
  • Author(s): 西山智明・鎌田寛彬・宮田大輔・山口勝司・重信秀治・坂山英俊・笠原雅弘
  • Organizer: 日本植物分類学会第17回大会
• [Presentation] シャジクモの生態型の進化に関する研究 (2017)
  • Author(s): 坂山英俊・宮田大輔・加藤 将・西山智明
  • Organizer: 日本藻類学会第41回大会
  • Place of Presentation: 高知大学（高知）
  • Year and Date: 2017-03-25
• [Presentation] 富士五湖における最近の車軸藻類の分布状況 (2017)
  • Author(s): 上嶋崇嗣・中村誠司・加藤 将・坂山英俊・芹澤(松山)和世・芹澤如比古
  • Organizer: 日本藻類学会第41回大会
  • Place of Presentation: 高知大学（高知）
  • Year and Date: 2017-03-24
• [Presentation] 分離集団の薄いショットガンシーケンシングによるシャジクモ遺伝学的地図の構築 (2017)
  • Author(s): 西山智明・鎌田寛彬・宮田大輔・山口勝司・重信秀治・坂山英俊・笠原雅弘
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島）
  • Year and Date: 2017-03-16
• [Presentation] 精子を持つ植物で見つかったアデニル酸シクラーゼ (2017)
  • Author(s): 笠原賢洋・末次憲之・浦野裕貴・山本千愛・大森未樹矢・高田侑季・奥田修二郎・西山智明・坂山英俊・河内 孝之・高橋文雄
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島）
  • Year and Date: 2017-03-16
• [Presentation] De novo sequencing of Chara braunii genome and construction of genetic map (2017)
  • Author(s): Nishiyama, T., Kamada, H., Miyata, D., Kasahara, M. and Sakayama, H.
  • Organizer: Advanced Genome Science International Symposium, The Start of New Genomics
  • Place of Presentation: 伊藤国際学術研究センター（東京）
  • Year and Date: 2017-01-10
  • Int'l Joint Research
• [Presentation] The genome and ecological evolution of a charalean alga Chara braunii (2017)
  • Author(s): Sakayama, H. and Nishiyama, T.
  • Organizer: 65th NIBB Conference Marchantia Workshop 2017
  • Int'l Joint Research / Invited
• [Presentation] Genetic map construction with low depth segregant sequencing in Chara braunii (2017)
  • Author(s): Nishiyama, T, Kamada H., Miyata D., Yamaguchi K., Shigenobu S., Sakayama H. and Kasahara M.
  • Organizer: 65th NIBB Conference Marchantia Workshop 2017
  • Int'l Joint Research
• [Presentation] 緑色植物の活性酸素生成酵素NADPH oxidase/Rbohの分子進化におけるCa2+依存的活性制御機構の獲得起源 (2017)
  • Author(s): 板橋 武・橋本研志・坂山英俊・西山智明・北畑信隆 ・Bonnot Clemence・Hetherington Sandy・Dolan Liam・朽津和幸
  • Organizer: 日本植物学会第81回大会
• [Presentation] 高速化ミオシンXI導入による単子葉植物ブラキポディウムの表現型解析 (2017)
  • Author(s): 原口武士・木下佳菜・玉那覇正典・坂山英俊・西山智明・富永基樹・伊藤光二
  • Organizer: 日本植物学会第81回大会
• [Presentation] シャジクモの生殖器官のトランスクリプ トーム解析 (2016)
  • Author(s): 西山智明・坂山英俊
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄）
  • Year and Date: 2016-09-16
  • Invited
• [Presentation] シャジクモの発生遺伝子の発現プロファイリングに関する研究 (2016)
  • Author(s): 渡邊みゆき・西山智明・鈴木 穣・坂山英俊
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄）
  • Year and Date: 2016-09-16
• [Presentation] 速度改変型ミオシン XI 導入による単子葉植物ブラキポ ディウムの成長促進 (2016)
  • Author(s): 原口武士・木下佳菜・玉那覇正典・坂山英俊・西山智明・富永基樹・伊藤光二
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄）
  • Year and Date: 2016-09-16
• [Presentation] 生物界最速ミオシンである Chara braunii (シャジクモ)のミオシンXIの機能解析 (2016)
  • Author(s): 玉那覇正典・原口武士・木下佳菜1・坂山英俊・西山智明・富永基樹・伊藤光二
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄）
  • Year and Date: 2016-09-16
• [Presentation] 藻類・陸上植物の細胞壁空間に ROS を産生する酵素 Rboh の分子進化 (2016)
  • Author(s): 板橋武・橋本研志・坂山英俊・西山智明・北畑信隆・Bonnot Clemence・Hetherington Sandy・Dolan Liam・朽津和幸
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄）
  • Year and Date: 2016-09-16
• [Presentation] Genome and transcriptome of charophytes (2016)
  • Author(s): Sakayama, H. & Nishiyama, T.
  • Organizer: 7th International Research Group on Charophytes Symposium
  • Place of Presentation: Astana, Kazakhstan
  • Year and Date: 2016-08-31
  • Int'l Joint Research
• [Presentation] Charophytes, origin of land plants and terminology (2016)
  • Author(s): Garcia, A. & Sakayama, H.
  • Organizer: 7th International Research Group on Charophytes Symposium
  • Place of Presentation: Astana, Kazakhstan
  • Year and Date: 2016-08-31
  • Int'l Joint Research
• [Presentation] Genomes of bryophytes and charophyceans (2016)
  • Author(s): Nishiyama, T., Sakayama, H, & Sakakibara, K.
  • Organizer: ICAR 2016 International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research / Invited
• [Presentation] Evolutionary analyses on ecological differentiation of a cosmopolitan freshwater alga Chara braunii (Charales, Streptophyta) (2016)
  • Author(s): Sakayama, H., Miyata, D., Kato, S. & Nishiyama, T.
  • Organizer: EMBO Workshop, New model systems for early land plant evolution
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] Genome sequencing charophyceans, liverworts, and hornworts: making model systems (2016)
  • Author(s): Nishiyama, T., Sakayama, H, Sakakibara, K, et al.
  • Organizer: EMBO Workshop, New model systems for early land plant evolution
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-06-22
  • Int'l Joint Research / Invited
• [Presentation] 水生植物の気孔形成と塩ストレス応答 (2016)
  • Author(s): 飯田聡子・坂山英俊・角野康郎・小菅 桂子
  • Organizer: 日本生態学会第63回大会
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-03-24
• [Presentation] 日本とオーストラリアから発見された車軸藻類シャジクモ属の新種の形態，系統，分類 (2016)
  • Author(s): 坂山英俊・Michelle T. Casanova・Kenneth G. Karol・加藤 将・樋口澄男・野崎久義・川井浩史
  • Organizer: 日本藻類学会第40回大会
  • Place of Presentation: 日本歯科大学（東京都・千代田区）
  • Year and Date: 2016-03-20
• [Presentation] Evolutional analysis of phosphate transport mechanism in plant cells (2016)
  • Author(s): Fujiwara, H., Sakayama, H., Ohnishi, M., Ishizaki, K., Toyokura, K., Goh, T., Sekimoto, H., Nishiyama, T., Ikegaya, H., Kanno, S., Tezuka, A., Nagano, A. J., Kosuge, K., Fukaki, H. and Mimura, T.
  • Organizer: 日本植物生理学会第57回年会
  • Place of Presentation: 岩手大学（岩手県・盛岡市）
  • Year and Date: 2016-03-19
• [Presentation] 水生植物の異形葉形成と海水適応 (2015)
  • Author(s): 飯田聡子・坂山英俊・角野康郎・小菅 桂子
  • Organizer: 近畿植物学会講演会（第4回）
  • Place of Presentation: 大阪市立大学理学部附属植物園（大阪府・交野市）
  • Year and Date: 2015-11-07
• [Presentation] シャジクモの遺伝学的解析に向けた交配実験系の確立 (2015)
  • Author(s): 宮田大輔・西山智明・川井浩史・坂山英俊
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-08
• [Book] 車軸藻類．京都府レッドデータブック 2015 第2巻 (野生植物・菌類編) (2015)
  • Author(s): 加藤 将・坂山英俊
  • Publisher: 京都府自然環境保全課