| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Molecular phylogenetic analyses of Urochordates based on the evolution of urochordate mitochondrial genetic system were performed. Appendicularian mitochondrial genomes were targets for the sequencing determination in this study. However, no tRNA and rRNA genes were found on the appendicularian mitochondrial genome sequences determined. In addition, we performed molecular phylogenetic analysis based on the mitochondrial protein gene sequences with Bayesian method under the CAT model. Appendicularians appeared to form monophyletic group with Enterogonan ascidians, although fast-evolving appendicularians and sales (Thaliacea, Salpida) tend to form a group in molecular phylogenetic trees (long branch attrition). Furthermore, the expression of Holocynthia roretzi (Ascidiacea, Enterogona) mitochondrial tRNAs had been analyzed by synthesizing cDNAs of these tRNAs.
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