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Sperm competition occurred in spawning of masu salmon

Research Project

Project/Area Number 15K07229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionNihon University

Principal Investigator

MAKIGUCHI Yuya  日本大学, 生物資源科学部, 講師 (00584153)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords代替繁殖戦略 / サクラマス / 精子運動 / 精子競争 / 体腔液 / 受精 / 乱婚 / サケ科魚類 / 生活史二型
Outline of Final Research Achievements

Masu salmon exhibits typical fixed male alternative reproductive tactics. The large anadromous males fight before spawning to achieve dominant access to the females, whereas the small parr males adopt a sneaker tactic to dart into spawning events and furtively position themselves under ovipositing females to release milt near the female as she releases her eggs and ovarian fluid. Sperm competition theory predicts that milt from multiple males enhance the fertilization competition because serum included in milt affect the sperm velocity, which is one of the important factor to determine the success of fertilization. The objective of this study is to investigate effect mixture of milt from parr males and anadromous males on hatching rate of eggs. Hatching rate was higher in mixture of parr and anadromous males than in same tactics group. Our results indicated that mixture of milt from parr and anadromous males improve the quality of hatching eggs.

Academic Significance and Societal Importance of the Research Achievements

本研究では精子速度が精漿や体腔液などの生体由来の成分に影響を受けることを明らかにした。精子運動性能、特に精子遊泳速度が雄の繁殖成功に大きな影響を与える要因として考えられて来た(Gage et al., 2004)。しかし、過去に測定された魚類の精子遊泳速度は河川水または海水などの無機的な溶媒を用いたものが大半であった。体外受精を行う魚類の受精環境では競争雄の精漿や雌の体腔液が多くの場合存在しており、これまで測定されてきた精子速度とは異なる可能性がある。本研究課題は、精漿が雄の繁殖成功に寄与する可能性に着眼している点が独創的であり行動生態学研究に新しい知見をもたらすことが期待される。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (5 results) Book (2 results)

  • [Int'l Joint Research] University of Windsor(Canada)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Reproductive investment patterns and comparison of sperm quality in the presence and absence of ovarian fluid in alternative reproductive tactics of masu salmon, Oncorhynchus masou2017

    • Author(s)
      Makiguchi, Y. Torao, M. Kojima, T. Pitcher, T. E.
    • Journal Title

      Theriogenology

      Volume: 86 Issue: 9 Pages: 2189-2193

    • DOI

      10.1016/j.theriogenology.2016.07.009

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Short term effects of relative tag size and surgical implantation on feeding behaviour, survival rate, plasma lactate and growth rate in juvenile to adult rainbow trout (Oncorhynchus mykiss)2017

    • Author(s)
      Makiguchi, Y., Kojima, T.
    • Journal Title

      Fisheries Research

      Volume: 185 Pages: 54-61

    • DOI

      10.1016/j.fishres.2016.09.035

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sperm allocation in relation to female size in a semelparous salmonid.2017

    • Author(s)
      Makiguchi, Y., Ichimura M, Kitayama T, Kawabata Y, Kitagawa T, Kojima T, Pitcher TE.
    • Journal Title

      Proceedings of the Royal Society of London, Open Science

      Volume: 3 Issue: 12 Pages: 160497-160497

    • DOI

      10.1098/rsos.160497

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Sex difference in metabolic rate and swimming performance in pink salmon (Oncorhynchus gorbuscha): the effect of male secondary sexual traits2016

    • Author(s)
      Makiguchi Y, Nii H, Nakao K, Ueda H
    • Journal Title

      Sex difference in metabolic rate and swimming performance in pink salmon (Oncorhynchus gorbuscha): the effect of male secondary sexual traits

      Volume: 2016 Issue: 2 Pages: 1-11

    • DOI

      10.1111/eff.12278

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] サクラマスの老魚と若魚における精子運動性能2018

    • Author(s)
      牧口祐也、泊健太、小島隆人
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 照度の違いがシロザケの求愛行動に与える影響2018

    • Author(s)
      横森洋治郎、牧口祐也、小島隆人
    • Organizer
      平成29年度日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 雌の体腔液がサクラマスの精子運動に与える影響と代替繁殖戦略の違いによる比較2017

    • Author(s)
      牧口祐也
    • Organizer
      第28回魚類生態研究会
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] ヒメマスの二次性徴とオスの精子運動性能の関係2017

    • Author(s)
      大村風音、牧口祐也、小島隆人
    • Organizer
      平成28年度日本水産学会春季大会
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] サクラマスの雌が乱婚から享受する利益の検証2017

    • Author(s)
      牧口祐也
    • Organizer
      第41回 長崎最西端 進化生態学セミナー
    • Related Report
      2017 Research-status Report
  • [Book] 生物の科学 遺伝2018

    • Author(s)
      牧口祐也
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2018 Annual Research Report
  • [Book] バイオロギング2 動物たちの知られざる世界を探る2016

    • Author(s)
      牧口祐也
    • Publisher
      京都通信社
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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