| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Oka reported that epistatic interaction between Hwa1-1and Hwa2-1 cause a hybrid weakness of rice and we have shown that these causal genes located on the almost the same region of chromosome 11. This research project aimed at molecular cloning of Hwa1-1and Hwa2-1. DNA sequence of the donor plants of these two genes were analyzed by PacBio DNA sequencers. DNA sequences of DNA markers used in fine mapping of these two causal genes were used as queries of homology search against DNA sequences derived from the donor plants using software BLAST (NCBI). As a result, a contig of Hwa1-1 was constructed and it had 20 kbp deletion in comparison with the Nipponbare genome sequence. On the other hand, the chromosomal structure of Hwa2-1region had large difference with that of Nipponbare homologous region, and we could not construct a contig covering the Hwa2-1interval.
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