| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this research, I aim to establish " a simple and versatile haploid production method". The "Haploid breeding" by "Haploid Production and Its Doubling" can greatly shorten the time to obtain the pure line. However, so far haploids have been produced by pollen cultivation or crossbreeding, and only a few species can utilize these methods. In this study, kinetochore proteins and centromeric DNA sequences were modified by genome editing in rice, Arabidopsis thaliana, tomato, and Lotus japonicus to decrease its function, and crossing the gametes having this low-functional centromere and the normal centromere will cause selectively eliminate chromosome sets derived from one parent in fertilized embryos and efficiently produce haploid.
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