Development of haploid production method using genome editing

Research Project

Project/Area Number	15K07256
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Science in genetics and breeding
Research Institution	Okayama University
Principal Investigator	NAGAKI Kiyotaka 岡山大学, 資源植物科学研究所, 准教授 (70305481)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	動原体 / 半数体
Outline of Final Research Achievements	In this research, I aim to establish " a simple and versatile haploid production method". The "Haploid breeding" by "Haploid Production and Its Doubling" can greatly shorten the time to obtain the pure line. However, so far haploids have been produced by pollen cultivation or crossbreeding, and only a few species can utilize these methods. In this study, kinetochore proteins and centromeric DNA sequences were modified by genome editing in rice, Arabidopsis thaliana, tomato, and Lotus japonicus to decrease its function, and crossing the gametes having this low-functional centromere and the normal centromere will cause selectively eliminate chromosome sets derived from one parent in fertilized embryos and efficiently produce haploid.