Studies on the crosstalk between brassinosteroid and jasmonate signaling in rice

Research Project

Project/Area Number	15K07263
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Science in genetics and breeding
Research Institution	Ishikawa Prefectural University
Principal Investigator	Sakamoto Tomoaki 石川県立大学, 生物資源環境学部, 准教授 (00345183)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	イネ / ブラシノステロイド / ジャスモン酸 / 情報伝達
Outline of Final Research Achievements	ERECT LEAF 1 (ELF1) was identified as a component of brassinosteroid signaling in rice. A double mutant obtained by crossing elf1 with the brassinosteroid receptor mutant d61 did not show epistasis Microarray analysis showed that fewer than half of genes differentially expressed between the wild-type and elf1 showed similar differences in d61 relative to the wild-type. These results indicate that less than half of ELF1-regulated genes in rice seedlings are affected by the receptor, and suggest that ELF1 acts in a rice brassinosteroid signaling pathway different from that initiated by the receptor. Gene expression analysis showed that some stress response-related genes were induced in elf1 but not in d61, and 8 of 9 genes oppositely regulated in elf1 and d61 were significantly up- or down-regulated in both elf1 and jasmonate-treated wild-type. These results suggest that ELF1 may be involved in the brassinosteroid-mediated suppression of jasmonic acid response in rice.