| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
ERECT LEAF 1 (ELF1) was identified as a component of brassinosteroid signaling in rice. A double mutant obtained by crossing elf1 with the brassinosteroid receptor mutant d61 did not show epistasis Microarray analysis showed that fewer than half of genes differentially expressed between the wild-type and elf1 showed similar differences in d61 relative to the wild-type. These results indicate that less than half of ELF1-regulated genes in rice seedlings are affected by the receptor, and suggest that ELF1 acts in a rice brassinosteroid signaling pathway different from that initiated by the receptor. Gene expression analysis showed that some stress response-related genes were induced in elf1 but not in d61, and 8 of 9 genes oppositely regulated in elf1 and d61 were significantly up- or down-regulated in both elf1 and jasmonate-treated wild-type. These results suggest that ELF1 may be involved in the brassinosteroid-mediated suppression of jasmonic acid response in rice.
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