| Project/Area Number |
15K07267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Fukuoka Agricultural and Forestry Research Center |
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Principal Investigator |
Wada Takuya 福岡県農林業総合試験場, 生産環境部, チーム長 (90502435)
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| Co-Investigator(Kenkyū-buntansha) |
熊丸 敏博 九州大学, 農学研究院, 教授 (00284555)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | イネ / QTL / タンパク質 / 遺伝子発現 / 遺伝育種科学 / 品質 / 水稲 / 種子 |
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| Outline of Final Research Achievements |
We validated the effect of QTLs for protein content of brown rice, which is related to eating quality of cooked rice, and tried to identify the responsible genes of those QTLs. The near isogenic lines harboring ‘Koshihikari’ allle of QTL for protein content, which is located in chromosome 2 (qPC2), decreased the protein content of brown rice at approximately 0.5%, compared to the recurrent parent cultivar. The NIL also showed heavier grain weight
The analysis using backcrossed population and micro-array analysis revealed that qPC2 was located in the lower position of candidate regions (around 30Mb region of chromosome 2). We also performed protein fraction analysis, but did not detect any significant difference of prolamin / glutelin ratio. These facts suggested that major QTL for protein content was located around 30Mb region on chromosome 2, and the QTL increased grain weight and consequently decreased protein content in rice grain.
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