| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The expression level of a Rubisco small subunit gene, OsRBCS3, increased approximately 15-fold by the supply of nitrogen. The increase in the expression level of OsRBCS5 gene was about 2-fold. This is because the degree of increase in transcription activity by nitrogen is larger in OsRBCS3 than OsRBCS5. In OsRBCS3, the increase in trimethylation level of the fourth lysine from the N terminus of histone H3 and acetylation level of the ninth lysine, which are involved in the activation of transcription, by nitrogen was greater than OsRBCS5. On the other hand, the dimethylation level of the ninth lysine from the N terminus of histone H3, which is involved in transcription suppression, was lowered by supply of nitrogen in OsRBCS3, while it did not change in OsRBCS5. These results suggest that the nitrogen expression of the OsRBCS3 and OSRBCS5 genes is regulated by the degree of modification of the histone H3.
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