| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Starch accumulated in rice stems and leaves before heading is degraded at the post-heading stage and utilized for the grain filling. In the present study, the repressed expression of both OsBAM2 and OsBAM3 encoding the β-amylase isoforms inhibited the decrease in starch content in the leaf sheaths at the post-heading stage. The transient expression of chimeric protein with GFP indicated that the other β-amylase isogene, OsBAM5, encode plastid-targeted protein. In addition, the repression of OsBAM5 expression caused excess accumulation of starch in the leaf sheaths after heading. Triple knockdown lines for OsBAM2, OsBAM3 and OsBAM5 were generated in Nipponbare. Furthermore, the knockdown lines of RAmy2A, an α-amylase isogene, which is highly expressed in the leaf sheaths of high yield variety Takanari, were successfully generated in Takanari.
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