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Functional analysis of the genes involved in starch degradation in rice leaves and stems and their varietal differences.

Research Project

Project/Area Number 15K07276
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Crop production science
Research InstitutionMeijo University

Principal Investigator

Hirano Tatsuya  名城大学, 農学部, 教授 (30319313)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsイネ / 葉鞘 / デンプン分解 / α-アミラーゼ / β-アミラーゼ / β-アミラーゼ / α-アミラーゼ
Outline of Final Research Achievements

Starch accumulated in rice stems and leaves before heading is degraded at the post-heading stage and utilized for the grain filling. In the present study, the repressed expression of both OsBAM2 and OsBAM3 encoding the β-amylase isoforms inhibited the decrease in starch content in the leaf sheaths at the post-heading stage. The transient expression of chimeric protein with GFP indicated that the other β-amylase isogene, OsBAM5, encode plastid-targeted protein. In addition, the repression of OsBAM5 expression caused excess accumulation of starch in the leaf sheaths after heading. Triple knockdown lines for OsBAM2, OsBAM3 and OsBAM5 were generated in Nipponbare. Furthermore, the knockdown lines of RAmy2A, an α-amylase isogene, which is highly expressed in the leaf sheaths of high yield variety Takanari, were successfully generated in Takanari.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Two β-amylase genes, OsBAM2 and OsBAM3, are involved in starch remobilization in rice leaf sheaths2016

    • Author(s)
      Tatsuya Hirano, Takayuki Higuchi, Minako Hirano, Yu Sugimura, Hiroyasu Michiyama
    • Journal Title

      Plant Production Science

      Volume: 19 Issue: 2 Pages: 291-299

    • DOI

      10.1080/1343943x.2016.1140008

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of α-amylase genes in starch degradation in rice leaf sheaths at the post-heading stage2015

    • Author(s)
      Yu Sugimura, Hiroyasu Michiyama and Tatsuya Hirano
    • Journal Title

      Plant Production Science

      Volume: 18 Pages: 277-283

    • NAID

      130005087242

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] イネ葉鞘において出穂期以降のデンプン分解に関与する遺伝子の探索と機能解析2017

    • Author(s)
      平野達也、杉村優有、樋口貴之
    • Organizer
      日本作物学会第243回講演会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] イネ葉鞘におけるデンプン含量の変化とα-グルカンホスホリラーゼ活性との関係2016

    • Author(s)
      樋口貴之、道山弘康、平野達也
    • Organizer
      日本作物学会第242回講演会
    • Place of Presentation
      大津
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] β-アミラーゼ遺伝子, OsBAM5の発現抑制系統はイネ葉鞘においてデンプン過剰の表現型を示す2015

    • Author(s)
      杉村優有、平野美奈子、道山弘康、深山浩、平野達也
    • Organizer
      日本作物学会第240回講演会
    • Place of Presentation
      長野
    • Year and Date
      2015-09-05
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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