| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Exposure of ripening rice caryopses to high temperature impairs their grain filling and produces low-quality chalky grains through imperfect filling with storage starch. Spatial expression pattern of starch-lytic α-amylase genes in the developing seeds as well as changes in grain appearance by ectopic overexpression of each of the genes revealed that expression of Amy1A, Amy3C and Amy3D was induced in the developing endosperm by the elevated temperature and that those isoforms degraded storage starch to produce the chalky grains. Screening of a yeast one-hybrid library consisting of rice transcription factors with a cis region in the Amy3C promoter identified bZIP61 as a candidate in regulation of its expression. Since overexpression of bZIP61 generated chalky grains, it would be a novel gene controlling grain quality.
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