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Developement of lighting treatment to extend day length for increasing the chlorogenic acid content in lettuce plant

Research Project

Project/Area Number 15K07284
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionUniversity of Tsukuba

Principal Investigator

FUKUDA Naoya  筑波大学, 生命環境系, 准教授 (80251023)

Co-Investigator(Kenkyū-buntansha) 吉田 英生  筑波大学, 生命環境系, 助教 (40729852)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsクロロゲン酸 / 植物工場 / レタス / 連続照明 / 青色光 / LED / シキミ酸経路 / メタボローム解析 / PAL活性 / メタボローム / カフェ酸 / DPPH / 抗酸化能力 / 根域ストレス / レタス類 / クロロゲン酸蓄積 / 機能性 / 根域制限 / 二酸化炭素処理 / 塩類ストレス / 高蓄積 / 二酸化炭素濃度
Outline of Final Research Achievements

Chlorogenic acid (CGA) is one of the polyphenol compounds and its health functions are expected because of the high antioxidant capacity. In this study, as we have focused on the CGA accumulation effect in lettuce plants grown under long day condition, and we tried to confirm the environmental factors to induce the higher CGA accumulation in lettuce plants for developing a new cultivation technologies to produce high CGA content lettuce in plant factory system. In the growth cabinet, the combination of continuous lighting, blue light irradiation, high CO2 concentration and root volume restriction treatment could induce the high CGA content, approximately up to 200 mg 100 gFW-1, in young lettuce plants. From results of metabolome analysis, it was shwon that in the case of a young lettuce plants, the CGA accumulation conditions could give a strong stimulus for the shikimic acid pathway on the synthesis and accumulation of CGA.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report

Research Products

(6 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Increase in Chlorogenic Acid Concentration in Lettuce by Overnight Supplemental Lighting and CO2 Enrichment2016

    • Author(s)
      H. Yoshida, K. Sekiguchi, L. Okushima, S. Sase and N. Fukuda
    • Journal Title

      Acta Horticulturae

      Volume: 印刷中

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 赤色光または青色光照射下で栽培したレタス幼植物体中のクロロゲン酸含有量日間変動と代謝物解析2018

    • Author(s)
      下村未知瑠・草野都・藤内直道・有泉亨・江面浩・福田直也
    • Organizer
      園芸学会平成30年度春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] レタス幼植物体に含まれるクロロゲン酸含量は根域環境と光周期によって変動する2017

    • Author(s)
      下村未知瑠・藤内直道・吉田英生・有泉亨・江面浩・福田直也
    • Organizer
      園芸学会平成29年度秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 青色光による連続照明ならびに二酸化炭素施用処理はレタス幼植物体においてクロロゲン酸蓄積を促進する2016

    • Author(s)
      下村未知瑠、吉田英生、有泉亨、江面浩、福田直也
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学天白キャンパス(愛知県・名古屋市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] Increase in Chlorogenic Acid Concentration in Lettuce by Overnight Supplemental Lighting and CO2 Enrichment2016

    • Author(s)
      H. Yoshida, K. Sekiguchi, L. Okushima, S. Sase and N. Fukuda
    • Organizer
      8th International Symposium on Light in Horticulture
    • Place of Presentation
      アメリカ合衆国 ミシガン州
    • Year and Date
      2016-05-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] キク科植物のクロロゲン酸含有量を増加させる方法およびキク科植物2016

    • Inventor(s)
      福田直也、吉田英生、下村未知瑠
    • Industrial Property Rights Holder
      筑波大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-09-08
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-05-15  

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