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Exhaustive analysis of cell cycle-related genes involving arrest of stamen primordia development of female flowers in cucumber sex expression

Research Project

Project/Area Number 15K07292
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionFukuoka University of Education

Principal Investigator

Yamasaki Seiji  福岡教育大学, 教育学部, 教授 (30363295)

Research Collaborator YAMAKUCHI Ryusei  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsキュウリ / 性分化 / 細胞周期関連遺伝子 / サイクリン / サイクリン依存性キナーゼ
Outline of Final Research Achievements

We found five cyclin-related genes and two cyclin-dependent kinases (CDKs) genes from data base of cucumber (Cucumis sativus L.) genome. Two cyclin-related genes were expressed specifically in the area containing arrested stamen primordia (ACASP) in female flower buds, but was barely detected in male flower buds in monoecious cucumber. Expression of the other five genes was detected in the ACASP but not in pistils of female flower buds. Expression of all seven genes was elevated 2-5 times within 6 hours transiently following the application of ethephon, an ethylene-releasing agent, to the shoot apices. These results suggest that these seven cell cycle-related genes play a role in the arrest of stamens of female flowers in monoecious cucumber. The mechanism that cell growth is restrained and finally cells die although cell division rates remained high in ACASP of female flowers should be clarified.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Potential Involvement of Cell Cycle-Related Genes in the Arrest of Stamen Development of Female Flowers During Sex Expression in Cucumber (<i>Cucumis sativus</i> L.)2017

    • Author(s)
      Yamasaki, S., Yamakuchi, R., Yamanaka, S., Manabe, K.
    • Journal Title

      Environment Control in Biology

      Volume: 55 Issue: 2 Pages: 105-112

    • DOI

      10.2525/ecb.55.105

    • NAID

      130005607977

    • ISSN
      1880-554X, 1883-0986
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] キュウリの雄花・雌花の蕾における細胞死関連遺伝子の発現解析2017

    • Author(s)
      山口流正,山中早織,山﨑聖司
    • Organizer
      日本生物環境工学会2017年松山大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] キュウリの性分化における細胞周期関連遺伝子群の発現解析.2016

    • Author(s)
      山口流正,山中早織,山﨑聖司
    • Organizer
      日本生物環境工学会
    • Place of Presentation
      金沢工業大学野々市キャンパス
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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