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Construction of a High-Density Linkage Map for loquat Using RAD-Seq and development of DNA markers on Fruit traits

Research Project

Project/Area Number 15K07293
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionSaga University

Principal Investigator

Shinji FUKUDA  佐賀大学, 農学部, 准教授 (70503770)

Co-Investigator(Kenkyū-buntansha) 永野 幸生  佐賀大学, 総合分析実験センター, 准教授 (00263038)
Co-Investigator(Renkei-kenkyūsha) YAMAMOTO Toshiya  国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 上席研究員 (60355360)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsビワ / 連鎖地図 / QTL解析 / 育種 / DNAマーカー / 果実形質 / 熟期 / 抵抗性
Outline of Final Research Achievements

We constructed a high-density genetic linkage map of bronze loquat for a three-way cross of loquat × 78-51by using single nucleotide polymorphism markers generated by RAD-Seq, with the combination of simple sequence repeat markers, which have been used in the previous study. The map of bronze loquat consisted of 960 loci including 793 SNPs, 166 SSRs (98 apple, 8 loquat and 60 pear SSRs), and the loquat canker resistance gene Pse-a. This map covered 17 linkage groups over a total length of 1707.4 cM with an average distance of 1.78 cM between markers. The number of linkage groups corresponded to the basic chromosome number. The analysis of the markers that can be anchored to apple draft genome showed that almost all loquat linkage groups were aligned to the apple draft genome. The constructed map will enable us to determine the location of quantitative trait loci and genes of interest, and to analyze genome synteny in the tribe Pyreae, subfamily Spiraeoideae of the family Rosaceae.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers2017

    • Author(s)
      Fukuda Shinji、Ishimoto Keiichiro、Sato Shusei、Terakami Shingo、Hiehata Naofumi、Yamamoto Toshiya
    • Journal Title

      Tree Genetics & Genomes

      Volume: 12 Issue: 4 Pages: 80-80

    • DOI

      10.1007/s11295-016-1040-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RAD-Seq法を用いたビワ高密度連鎖地図の作製2017

    • Author(s)
      松隈公孝、石本慶一郎、稗圃直史、山本俊哉、永野幸生、永野惇、福田伸二
    • Journal Title

      園芸学研究

      Volume: 16(1) Pages: 293-293

    • Related Report
      2017 Annual Research Report
  • [Journal Article] ビワの熟期に関する量的遺伝子座(QTLs)2017

    • Author(s)
      松隈公孝、中川利嗣、石本慶一郎、稗圃直史、山本俊哉、永野幸生、福田伸二
    • Journal Title

      園芸学研究

      Volume: 16(2) Pages: 144-144

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Genetic mapping of the loquat canker resistance gene pse-c in loquat(Eriobotrya japonica)2016

    • Author(s)
      Shinji Fukuda, Keiichiro Ishimoto, Shingo Terakami, Toshiya Yamamoto, Naofumi Hiehata
    • Journal Title

      Scientia Horticulturae

      Volume: 200 Pages: 19-24

    • DOI

      10.1016/j.scienta.2015.12.036

    • Related Report
      2017 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] RAD-seq法を用いたビワ高密度連鎖地図の作製2017

    • Author(s)
      松隈公孝・石本慶一郎・稗圃直史・山本俊哉・永野幸生・永野惇・福田伸二
    • Organizer
      園芸学会
    • Place of Presentation
      神奈川県藤沢市 日本大学
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] RAD-Seq法を用いたビワ高密度連鎖地図の作製2017

    • Author(s)
      松隈公孝、石本慶一郎、稗圃直史、山本俊哉、永野幸生、永野惇、福田伸二
    • Organizer
      園芸学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ビワの熟期に関する量的遺伝子座(QTLs)2017

    • Author(s)
      松隈公孝、中川利嗣、石本慶一郎、稗圃直史、山本俊哉、永野幸生、福田伸二
    • Organizer
      園芸学会
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2021-12-27  

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