| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
Gene expression analysis by RNA-Seq was conducted to investigate the flowering induction mechanism of Phalaenopsis by GA. When GA was applied at apical bud, flower-related genes such as SPL, AP2, FT, AP1 and GA biosynthesis and signal transduction genes such as GA20ox, GA3ox, GA2ox, GID1, DELLA were greatly different. The expression level of PhFT and ORAP13 at the shoot apex increased 840 fold and 8.3 fold at 7 weeks after the beginning of GA application compared with those of before GA application, respectively. Therefore, there are specific mechanism that PhFT was remarkably expressed at the shoot apex, followed by the expression level of ORAP13 was also increased during the flower induction at apical bud by GA.
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