| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Outline of Final Research Achievements |
Changes in miRNA accumulation and chromatin modification were analyzed during primed activation of AGO2 transcription by BTH treatment in Arabidopsis thaliana. RNA-seq analysis revealed that BTH treatment affected miRNA levels. The increased accumulation of miR403 and miR168 were also confirmed by northern blotting. The expression of AGO2 was constitutively primed in hyl1-2 and hst15 mutants. We also confirmed that the level of H3K4me3 was increased at transcription initiation point and -2,000 bp region of AGO2 promoter by BTH treatment. Binding activity of RNAPII was decreased at transcription initiation point of AGO2 in BTH-treated plants. Finally, we identified WRKY25 and AtHMGB9 as candidates of transcription factor which regulate primed activation of AGO2.
|
