| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. However, virus discovery in obligate biotrophs such as powdery mildews (ascomycetes) and rust fungi (basidiomycetes) is still very limited. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least nine novel totivirus (genus Totivirus) in this fungus. Similar totvirus-like sequences are found in public transcriptome shotgun assembly (TSA) libraries of the bean rust fungi. Our data suggests that the horizontal transmission of ancestral totiviruses might have occurred across quite different fungal phyla, between powdery mildews and rust fungi and later evolved within the new host fungus separately.
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