Virome of the phytopathogenic fungi: metagenomic analysis and the possible utilization
| Project/Area Number |
15K07312
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant protection science
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| Research Institution | Okayama University |
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Principal Investigator |
Kodno Hideki 岡山大学, 資源植物科学研究所, 准教授 (40263628)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 信弘 岡山大学, 資源植物科学研究所, 教授 (70206514)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ウイルス / 菌類 / メタゲノム解析 / 次世代シークエンサー / 絶対寄生菌 / うどんこ病菌 / クローバー / トティウイルス |
|---|
| Outline of Final Research Achievements |
The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. However, virus discovery in obligate biotrophs such as powdery mildews (ascomycetes) and rust fungi (basidiomycetes) is still very limited. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least nine novel totivirus (genus Totivirus) in this fungus. Similar totvirus-like sequences are found in public transcriptome shotgun assembly (TSA) libraries of the bean rust fungi. Our data suggests that the horizontal transmission of ancestral totiviruses might have occurred across quite different fungal phyla, between powdery mildews and rust fungi and later evolved within the new host fungus separately.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Evidence for a novel negative-stranded RNA mycovirus in the plant pathogenic fungus Fusarium graminearumn.2018
Author(s)
Wang, L., He, H., Chen, X., Qiu, D., Kondo, H.* and Guo, L.*
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Journal Title
Virology
Volume: 528
Pages: 232-240
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Reprint of ”Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi.”2016
Author(s)
Kondo, H., Hisano, S., Chiba, S., Maruyama K, Andika IB, Toyoda, K., Fujimori, F. and Suzuki, N.
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Journal Title
Virus Research
Volume: 219
Pages: 39-50
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] A novel betapartitivirus RnPV6 tolerate host RNA silencing but is interfered by its defective- RNAs.2016
Author(s)
Chiba, S., Lin, Y.-H., Zhang, Kondo, H., Kanematsu, S., and Suzuki, N.
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Journal Title
Virus Research
Volume: in press
Pages: 62-72
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi2016
Author(s)
Kondo, H., Hisano, S., Chiba, S., Maruyama K, Andika IB, Toyoda, K., Fujimori, F. and Suzuki, N.
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Journal Title
Virus Research
Volume: 213
Pages: 353-364
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A capsidless ssRNA virus hosted by an unrelated dsRNA virus2016
Author(s)
Zhang, R., Hisano, S., Tani, A., Kondo, H., Kanematsu, S., and Suzuki, N.
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Journal Title
Nature Nicrobiology
Volume: 1
Issue: 1
Pages: 15001-15001
DOI
NAID
Related Report
Peer Reviewed / Open Access
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