Analysis of virulence-related genetic region using Chinese yam necrotic mosaic virus infectious cDNA clone

• Project/Area Number: 15K07317
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Aomori Prefectural Industrial Technology Research Center
• Principal Investigator: Kondo Toru 地方独立行政法人青森県産業技術センター, 農林部門, 研究管理員 (70543908)
• Co-Investigator(Kenkyū-buntansha): 佐野 輝男 弘前大学, 農学生命科学部, 教授 (30142699)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ヤマノイモえそモザイクウイルス / 感染性クローン

Outline of Final Research Achievements

Chinese yam necrotic mosaic virus (CYNMV) occurs in Nagaimo plants in the world and causes severe yield losses. We had selected an attenuated CYNMV strain and demonstrated that Nagaimo plants infected with the attenuated CYNMV strain were effectively protected from subsequent CYNMV infections that occurred in the field, thus preventing a substantial yield loss. In this study, different genomic regions of the severe CYNMV strain were replaced with the corresponding fragments of the attenuated strain to create a series of recombinants throughout the viral genome. The infectivity of chimeric cDNA clone was analyzed by biolistic inoculation of Nagaimo plants. Our results suggested that mutations in BamHI-SpeI region of CYNMV genome might influence the pathogenicity and/or infectivity of CYNMV on Nagaimo plants.