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Identification and characterization of novel biocontrol factors from plant-protecting bacteria

Research Project

Project/Area Number 15K07318
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionNational Agriculture and Food Research Organization (2016-2017)
国立研究開発法人農業生物資源研究所 (2015)

Principal Investigator

Takeuchi Kasumi  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (40370663)

Research Collaborator SOMEYA Nobutaka  農業・食品産業技術総合研究機構, 野菜花き研究部門 野菜生産システム研究領域 生産環境ユニット, 上級研究員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords植物保護細菌 / バイオコントロール / シグナル伝達系 / 二次代謝産物 / 一次代謝産物 / ゲノム解析 / Pseudomonas属細菌 / 根圏 / 遺伝子発現制御 / 細菌 / ゲノム情報 / 植物根圏
Outline of Final Research Achievements

The Gram-negative species of Pseudomonas protegens are known as plant-protecting bacteria, as they suppress the growth of pathogenic microbes in rhizosphere by producing antimicrobial secondary metabolites via the Gac/Rsm signal transduction pathway. In this study, we identified the novel gene clusters which are involved in potential biocontrol activities. Furthermore, from the metabolome data obtained previous study, we suggest an important role of GABA in the Gac/Rsm-regulated niche adaptation of P. protegens CHA0 to plant roots.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016 2015 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] GABA, A Primary Metabolite Controlled by the Gac/Rsm Regulatory Pathway, Favors a Planktonic Over a Biofilm Lifestyle in Pseudomonas protegens CHA0.2018

    • Author(s)
      Kasumi Takeuchi
    • Journal Title

      Mol Plant Microbe Interact

      Volume: 31 Issue: 2 Pages: 274-282

    • DOI

      10.1094/mpmi-05-17-0120-r

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シュードモナス~基礎と応用をつなぐ2018

    • Author(s)
      竹内香純
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 26-30

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Pseudomonas protegens―植物の守護者と命名された細菌2017

    • Author(s)
      染谷信孝、諸星知広、竹内香純
    • Journal Title

      Soil Microorganisms

      Volume: 71 Issue: 2 Pages: 37-43

    • DOI

      10.18946/jssm.71.2_37

    • NAID

      130006188823

    • ISSN
      0912-2184, 2189-6518
    • Related Report
      2017 Annual Research Report
  • [Journal Article] Complete Genome Sequence of <i>Pseudomonas chlororaphis</i> subsp. <i>aurantiaca</i> Reveals a Triplicate Quorum-Sensing Mechanism for Regulation of Phenazine Production2017

    • Author(s)
      Morohoshi T, Yamaguchi T, Xie X, Wang WZ, Takeuchi K, Someya N.
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 1 Pages: 47-53

    • DOI

      10.1264/jsme2.ME16162

    • NAID

      130005519590

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] バイオコントロール細菌 Pseudomonas protegensの根圏における生存戦略2015

    • Author(s)
      竹内香純
    • Journal Title

      植物防疫

      Volume: 69 Pages: 374-376

    • NAID

      40020499394

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Presentation] ゲノムマイニングによるPseudomonas sp. Os17の抗菌性関連遺伝子の探索と解析2018

    • Author(s)
      竹内香純、土屋渉、染谷信孝、山崎俊正
    • Organizer
      平成30年度 日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物由来Pseudomonas chlororaphisの抗菌物質フェナジン生産におけるQuorum Sensing多重制御機構の解析2017

    • Author(s)
      諸星知広、山口貴人、謝肖男、竹内香純、染谷 信孝
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2017-03-23
    • Related Report
      2016 Research-status Report
  • [Presentation] GABAはPseudomonas protegensの浮遊状態を促進する2017

    • Author(s)
      竹内香純、Dieter Haas
    • Organizer
      平成29年度 日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 「植物の守り菌」-日本産Pseudomonas protegensの探索と機能解析2017

    • Author(s)
      染谷信孝、諸星知広、海野佑介、窪田昌春、竹内香純
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物保護効果を有するPseudomonas属細菌による抗菌物質生産制御機構の解析2017

    • Author(s)
      久勝順平、染谷信孝、竹内香純、諸星知広
    • Organizer
      第69回 日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Pseudomonas protegensのGac/Rsmを介した一次代謝制御2016

    • Author(s)
      竹内香純、Dieter Haas
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2016-03-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Survival strategies of plant-protecting bacteria in rhizosphere2016

    • Author(s)
      Kasumi Takeuchi
    • Organizer
      CFAI 2nd International Symposium, Current and Future Trends in Food and Agricultural Immunology
    • Place of Presentation
      東北大学(宮城県・仙台市)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparative genome analysis of the Pseudomonas protegens and closely related strains2015

    • Author(s)
      Kasumi Takeuchi
    • Organizer
      The Definitive Pseudomonas Workshop
    • Place of Presentation
      スイス
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 根圏より単離したPseudomonas protegens とその近縁系統の比較ゲノム解析によるバイオコントロール因子の同定2015

    • Author(s)
      竹内香純, 野田なほみ, 片寄裕一, 向井喜之, 沼寿隆, 山田小須弥, 染谷信孝
    • Organizer
      植物微生物研究会第25回研究交流会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-09-14
    • Related Report
      2015 Research-status Report
  • [Remarks] 農研機構 生物機能利用研究部門 植物微生物機能ユニット

    • URL

      http://www.naro.affrc.go.jp/nias/introduction/chart/0403/index.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 農業生物資源研究所 植物科学研究領域 植物・微生物間相互作用研究ユニット

    • URL

      http://www.nias.affrc.go.jp/org/DivPlant/Plant-Microbe/

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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