| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Ralstonia solanacearum injects virulence effector proteins into plant cells to succeed in infection. One of the effector proteins, RipAY, was found to function as a γ-glutamyl cyclotransferase, which degrade glutathione (GSH), a tripeptide that plays important roles in the plant immune system. The in vitro GSH degradation activity of RipAY is specifically activated by eukaryotic factors in the yeast and plant extracts. Interestingly, RipAY was activated by plant h-type thioredoxins that exist in large amounts in the plant cytosol in a thiol-independent manner. On the other hand, RipAY was not activated by bacterial thioredoxins. The high GSH degradation activity of RipAY is sufficient to suppress plant immunity during infection. However, GSH plays important roles in bacterial tolerance to various stresses and growth. The specific activation of RipAY in plant cells may be developed to prevent unwanted firing of its enzyme activity in bacterial cells.
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