Molecular mechanism for activation of Ralstonia solanacearum type III effector RipAY in plant cells
Project/Area Number |
15K07320
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | 岡山県農林水産総合センター生物科学研究所 |
Principal Investigator |
MUKAIHARA Takafumi 岡山県農林水産総合センター生物科学研究所, その他部局等, 専門研究員 (80344406)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | エフェクター / グルタチオン / 植物免疫 / 青枯病 / チオレドキシン / 病原因子 / 青枯病菌 / 病害抵抗性 / 病原菌 / 植物 |
Outline of Final Research Achievements |
Ralstonia solanacearum injects virulence effector proteins into plant cells to succeed in infection. One of the effector proteins, RipAY, was found to function as a γ-glutamyl cyclotransferase, which degrade glutathione (GSH), a tripeptide that plays important roles in the plant immune system. The in vitro GSH degradation activity of RipAY is specifically activated by eukaryotic factors in the yeast and plant extracts. Interestingly, RipAY was activated by plant h-type thioredoxins that exist in large amounts in the plant cytosol in a thiol-independent manner. On the other hand, RipAY was not activated by bacterial thioredoxins. The high GSH degradation activity of RipAY is sufficient to suppress plant immunity during infection. However, GSH plays important roles in bacterial tolerance to various stresses and growth. The specific activation of RipAY in plant cells may be developed to prevent unwanted firing of its enzyme activity in bacterial cells.
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Report
(4 results)
Research Products
(7 results)