| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Arabidopsis borate transporter BOR1 is localize to the inner plasma membrane in the root cell, and the polar localization is effective to transport boron which is the micronutrient of plant from soil to the plant above ground part effectively. We introduced the sequence domain that decided inward polar localization of BOR1 into the nutrient transporter except the BOR borare transporter. After fusing with the sequence of the BOR1-C-terminal domain in the PCR2 gene which was a heavy metal transporter, PCR2 showed inward polarity. This result shows possibility that the C-terminal domain sequence of BOR1 can be used as signal sequence for inward polar localization to various plasma membrane proteins.
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