Involvement of genistein-inducible Bradyrhizobium japonicum TetR family early in the interaction with Glycine max (L.) Merr

Research Project

Project/Area Number	15K07346
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied microbiology
Research Institution	Obihiro University of Agriculture and Veterinary Medicine
Principal Investigator	Ohwada Takuji 帯広畜産大学, 畜産学部, 教授 (90211804)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	TetR family / 多剤排出ポンプ / ゲニステイン / ダイズ根粒菌 / 相利共生 / 根粒菌多剤排出ポンプ / 共生 / ダイズ根粒菌TetR family
Outline of Final Research Achievements	The genistein-specific induction of Bradyrhizobium japonicum TetR family structural genes was caused by a repressor function. The multidrug efflux pump of the TetR family could be induced early in the symbiosis and functionally regulate the expression profiles of symbiosis genes by adjusting the intracellular genistein concentration by its efflux having cytotoxicity. The results showed a new paradigm that TetR family plays an important role in symbiosis.

Report

(4 results)