Analysis of the regulatory mechanism of the denitrification genes in an aerobic anoxygenic photosynthetic bacterium
| Project/Area Number |
15K07351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Arai Hiroyuki 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (70291052)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 脱窒 / 光合成 / 一酸化窒素 / 微生物 |
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| Outline of Final Research Achievements |
An aerobic anoxygenic photosynthetic bacterium Roseobacter denitrificans OCh114 requires two DNR-type transcriptional regulators, DNR1 and DNR2, for anaerobic growth by denitrification. Gene expression analyses using the mutant strains of some regulatory genes revealed that DNR1, DNR2, and the denitrifications genes constitute a cascade regulatory system. DNR1 regulates the expression of DNR2 and DNR2 directly regulates the expression of the denitrification genes in the system. An oxygen-responsive regulator FnrL was indirectly involved in the expression of the denitrification genes via regulation of the biosynthesis of heme, which is required for the activity of DNR. LOV-HK, which is involved in the regulation of the photosynthetic genes, was predicted to be involved in the light-responsive regulation of the denitrification genes in OCh114.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Dynamics of Nitric Oxide Controlled by Protein Complex in Bacterial System2017
Author(s)
Erina Terasaka, Kenta Yamada, Po-Hung Wang, Kanta Hosokawa, Raika Yamagiwa, Kimi Matsumoto, Shoko Ishii, Takaharu Mori, Kiyoshi Yagi, Hitomi Sawai, Hiroyuki Arai, Hiroshi Sugimoto, Yuji Sugita, Yoshitsugu Shiro and Takehiko Tosha
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Journal Title
PNAS
Volume: 114
Issue: 37
Pages: 9888-9893
DOI
Related Report
Peer Reviewed
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