| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The functions of protein kinase C (PKC) in filamentous fungi remain largely unknown. Aspergillus nidulans is one of the model filamentous fungus and there is a PKC-encoding gene, pkcA, in its genome. In our previous study, we showed that pkcA is essential for the growth of A. nidulans and that pkcA is involved in the cell wall integrity pathway and the establishment of cell polarity. In this study, to clarify the function of PkcA in the synthesis of secondary metabolites, we analyzed it using the pkcA-ts mutant in which the activity of PkcA was temperature sensitive and the R429A mutant that produces an activated form of PkcA under the control of the alcA promoter of A. nidulans. We identified a gene cluster that is involved in the production of a secondary metabolite using these strains.
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