| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
To gain insights into the localization mechanisms of membrane proteins to early or late Golgi in yeast, we analyzed the functions and localization of type II Golgi membrane proteins. We tagged the Mnt2 and Mnt3, mannose transferases whose localization mechanism to the Golgi have not been revealed so far, with 3xHA and analyzed the distribution between early and late Golgi cisternae and sought to find the binding proteins to them. Our analysis revealed that Svp26 functioned as ER exit adaptor of Mnt2 and Mnt3. Similar analyses revealed that Svp26 and Mnn6 facilitated the ER exit of Mnn4.
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