| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The E. coli knockout library was cultured in M9-0.5% glucose medium supplemented with high concentrations of spermidine and 14 strains with decreased acetylspermidine concentration outside the cells were found. However, transporters critical to acetylspermidine efflux could not be found. During this study, a novel transporter SapBCDF which releases putrescine was found.
E. coli deficients in the biosynthetic and catabolic pathways of putrescine was cultured in minimal medium containing high concentration of spermidine but not containing putrescine. Since putrescine was not detected from the culture supernatant nor from the cells, spermidine oxidase was not found under the condition used.
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