Analysis of the regulation for oxidative stress in cysteine metabolism related to Coenzyme Q
| Project/Area Number |
15K07360
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Shimane University |
|---|
Principal Investigator |
Kaino Tomohiro 島根大学, 生物資源科学部, 准教授 (90541706)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | Coenzyme Q / ubiquinone / cysteine / コエンザイムQ / ユビキノン / 酸化ストレス |
|---|
| Outline of Final Research Achievements |
The growth of fission yeast (S. pombe) CoQ deficient mutants required the addition of cysteine (Cys) or glutathione for antioxidant to the medium, but the affect for gene expression in CoQ deficient mutants have not been elucidated. When the gene expression of the CoQ deficient mutant was analyzed by microarray, the genes of transporter for ion or sulfur compounds were upregulated. The mutant of cysteine synthase gene show the sensitive for hydrogen peroxide and paraquat and the level of reactive oxygen species (ROS) were induced.
|
Report
(4 results)
Research Products
(51 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Breeding of recombinant fission yeast for high production of Coenzyme Q102016
Author(s)
D. Moriyama, T. Kaino, K. Hosono, M. Fujii, R. Yanai, K. Yajima, Y. Ikenaka, J. Hasegawa, M. Washida, H. Nanba, M. Kawamukai
Organizer
The Fifth International Conference on Cofactors (ICC-05) and Active Enzyme Molecule 2016 (AEM 2016)
Place of Presentation
Kurobe Unazuki International Hall Selene (Toyama)
Year and Date
2016-09-04
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
