Identification of the genes that involved in the selective production of citric acid by white koji mold Aspergillus kawachii
| Project/Area Number |
15K07362
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Saga University (2016-2017)
Kyushu University (2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小林 元太 佐賀大学, 農学部, 教授 (40291512)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Aspergillus / 麹菌 / 有機酸生産 / 遺伝子破壊 / ゲノム / Aspergillus kawachii / クエン酸生産 / イタコン酸生産 / オミックス解析 / RNA-seq解析 / 有機酸発酵 |
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| Outline of Final Research Achievements |
We attempted to elucidate the mechanism for hyperproduction of citric acid by white koji mold, Aspergillus kawachii which has been used for brewing shochu. Omics analyses allowed to predict at least 36 genes of A. kawachii that were candidate for the genes that are related with hyper and selective production of citric acid. Thirtysix genes were respectively disrupted in A. kawachii. The productivity of organic acids by the disruptants was compared to that by wt strain. We found the genes that affected the production ratio of citric acid and malic acid, the genes that affected the productivity of both citric acid and malic acid. Among 36 genes, the ctpA gene encoding mitochondrial citrate-malate transporter are in part associated with hyperproduction of citric acid by A. kawachii. We successfully introduced A. itaconicus cadA1 gene into A. kawachii, leading to producing valuable organic acid, itaconic acid.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Marker recycling system using the <i>sC</i> gene in the white koji mold, <i>Aspergillus luchuensis</i> mut. <i>kawachii</i>2016
Author(s)
Kadooka, C, Onitsuka, S, Uzawa, M, Tashiro, S, Kajiwara, Y, Takashita, H, Okutsu, K, Yoshizaki, Y, Takamine, K, Goto, M, Tamaki, H, Futagami, T.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 62
Issue: 3
Pages: 160-163
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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[Journal Article] Transcriptomic analysis of temperature responses of Aspergillus kawachii during barley koji production.2015
Author(s)
Futagami, T., Mori, K., Wada, S., Ida, H., Kajiwara, Y., Takashita, H., Tashiro, K., Yamada, O., Omori, T., Kuhara, S., and Goto, M.
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Journal Title
Appl. Environ. Microbiol.
Volume: 81
Issue: 4
Pages: 1353-1363
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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