| Project/Area Number |
15K07364
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
TAMAKI Hisanori 鹿児島大学, 農水産獣医学域農学系, 教授 (20212045)
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| Co-Investigator(Kenkyū-buntansha) |
二神 泰基 鹿児島大学, 農水産獣医学域農学系, 准教授 (60512027)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 細胞質分裂 / 脂質分解酵素 / 分裂酵母 / コンディショナルノックアウト / デグロン |
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| Outline of Final Research Achievements |
In this study, we tried to clarify the function of phospholipase Pah1 on cytokinesis in fission yeast. Since pah1 is essential gene, we constructed some pah1-conditional knock out strains. However, pah1 condisional knock out strain constructed such as (1)pah1 degron strain, (2)uracil regulatable pah1 strain, and (3)tetracycline regulated pah1 repressible strain did not work well, and thus functional analysis of Pah1 is not completed yet. To elucidate the function of pah1, pah1 temperature sensitive strain should be obtained.
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