| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Bacillus subtilis SPβ prophage is excised from the host chromosome in a process of sporulation to reconstitute a composite spsM. As results of in vitro recombination experiments, we revealed that the site-specific recombination occurred at the position of AA in the center of recombination site “5'-ACAGATAA /AGCTGTAT-3 '” catalyzed by an integrase SprA. Furthermore, we showed that a recombination directionality factor (RDF) SprB is necessary for synaptic complex formation between SprA and substrate DNA during excision while SprB inhibits the formation during integration. We also developed a detection system for B. subtilis dedifferentiated cells from mother cells and new genome modifying tool based on the SPβ recombination function.
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