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Elucidation of mechanism for lipid production in oleaginous yeast

Research Project

Project/Area Number 15K07372
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionNigata University of Phermacy and Applied Life Sciences

Principal Investigator

Takaku Hiroaki  新潟薬科大学, 応用生命科学部, 教授 (70350717)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords油脂酵母 / Lipomyces / 油脂蓄積変異株 / 遺伝子発現 / 遺伝子組換え / トリアシルグリセロール / 脂肪酸合成 / 油脂高蓄積変異株 / アセチルCoA / トリアシルグリセロール分解酵素 / ベータ酸化
Outline of Final Research Achievements

Biodiesel, one of next generation fuels, is mainly produced by transesterification of edible vegetable oils. The usage of edible oils as a biodiesel feedstock is often controversial because of the competition with food production. Therefore, we noted the oleaginous yeast Lipomyces starkeyi, which can convert non-edible biomass-derived carbohydrates to lipid. L. starkeyi is an excellent lipid producer that is able to accumulate up to 70% of its biomass as triacylglycerols. However, there are issues of lipid productivity for commercial applications.
In this study, we obtained of L. starkeyi mutants accumulating a high level of lipid. We extracted genes related to lipid biosynthesis by comparing gene expression profiles in the wild-type and mutants. The high-level expression of those extracted genes in the wild -type showed the high-level lipid production.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 2 results) Presentation (18 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains2017

    • Author(s)
      Yoshifumi Oguro, Harutake Yamazaki, Satoshi Ara, Yosuke Shida, Wataru Ogasawara, Masamichi Takagi and Hiroaki Takaku
    • Journal Title

      Current Genetics

      Volume: 印刷中 Issue: 4 Pages: 751-763

    • DOI

      10.1007/s00294-017-0679-6

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 油脂酵母Lipomyces starkeyiにおける遺伝子組換えシステムの構築とその応用2017

    • Author(s)
      高久洋暁、山崎晴丈
    • Journal Title

      オレオサイエンス

      Volume: 17 Pages: 107-116

    • NAID

      130007687593

    • Related Report
      2016 Research-status Report
    • Acknowledgement Compliant
  • [Presentation] 油脂酵母Lipomyces starkeyiの油脂低蓄積変異株の取得及びその油脂蓄積性の解析2018

    • Author(s)
      酒井 里佳子,荒 学志,山崎 晴丈,志田 洋介,小笠原 渉,矢追 克郎,荒木 秀雄,高久 洋暁
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 赤色油脂酵母Rhodosporidium toruloides の油脂高蓄積変異株の取得及びその油脂蓄積性の解析2018

    • Author(s)
      風間 春香,岡 由佳,小林 鈴花,荒 学志,山崎 晴丈,志田 洋介,小笠原 渉,矢追 克郎,森 一樹,油谷 幸代,荒木 秀雄,高久 洋暁
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脂質工学への展開を視野に入れた油脂酵母 Lipomyces starkeyi の簡易的形質転換系の開発2018

    • Author(s)
      宮島 温美,荒 学志,山崎 晴丈,志田 洋介,小笠原 渉,矢追 克郎,荒木 秀雄,高久 洋暁
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 油脂酵母 Lipomyces starkeyi の油脂超高蓄積変異株の取得及び油脂蓄積重要遺伝子の同定2018

    • Author(s)
      春日 琴葉,海老名 紗也佳,荒 学志,山崎 晴丈,志田 洋介,小笠原 渉,矢追 克郎,森 一樹,油谷 幸代,荒木 秀雄,高久 洋暁
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 油脂生産性向上へ向けた油脂酵母Lipomyces starkeyiの脂質工学的改変2018

    • Author(s)
      小林鈴花、荒学志、山崎晴丈、志田洋介、小笠原渉、矢追克郎、荒木秀雄、高久洋暁
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 油脂酵母 Lipomyces starkeyi の油脂高蓄積変異株の取 得と油脂合成・分解関連重要遺伝子の同定2017

    • Author(s)
      小林鈴花, 海老名沙也佳, 阿部史歩, 山崎晴丈, 志田洋介,小笠原渉,高久洋暁
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 油脂酵母 Lipomyces starkeyi における油脂高蓄積変異 株の育種2017

    • Author(s)
      海老名沙也佳, 春日琴葉, 志田洋介, 小笠原渉, 山崎晴丈,高久洋暁
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 油脂酵母 Lipomyces starkeyi における脂肪酸の分泌発 酵生産2017

    • Author(s)
      佐野真那理, 志田洋介, 小笠原渉, 山崎晴丈, 高久洋暁
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Isolation of Industrial Oleaginous yeast Lipomyces starkeyi Mutants Accumulating a High Level of Lipid2016

    • Author(s)
      Sayaka EBINA,Shiho ABE, Harutake YAMAZAKI, and Hiroaki TAKAKU
    • Organizer
      International Conference on Food for Health in Niigata 2016
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Metabolic engineering of oleaginous yeast Lipomyces starkeyi for over production and secretion of fatty acids from glucose2016

    • Author(s)
      Manari SANO, Harutake YAMAZAKI, and Hiroaki TAKAKU
    • Organizer
      International Conference on Food for Health in Niigata 2016
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Expression profile of genes responsible for lipid production in oleaginous yeast Lipomyces starkeyi mutant K13 exhibiting high lipid accumulation2016

    • Author(s)
      Suzuka KOBAYASHI, Harutake YAMAZAKI, and Hiroaki TAKAKU
    • Organizer
      International Conference on Food for Health in Niigata 2016
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 海老名沙也佳、阿部史歩、志田洋介、小笠原渉、山崎晴丈、高久洋暁2016

    • Author(s)
      Expression Profile of Genes Responsible for Lipid Production and Degradation on Oleaginous Yeast Lipomyces starkeyi mutants Accumulating a High Level of Lipid
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 有用産業油脂酵母Lipomyces starkeyiにおける油脂大量生産条件の検討2016

    • Author(s)
      小黒芳史、澤井隆明、山崎晴丈、志田洋介、小笠原渉、高久洋暁
    • Organizer
      バイオマス科学会議
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2016-01-20
    • Related Report
      2015 Research-status Report
  • [Presentation] 産業油脂酵母Lipomyces starkeyiの油脂高蓄積変異株の取得2016

    • Author(s)
      海老名沙也佳、志田洋介、小笠原渉、山崎晴丈、高久洋暁
    • Organizer
      バイオマス科学会議
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2016-01-20
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂酵母の油脂蓄積能向上へ向けて2016

    • Author(s)
      高久洋暁
    • Organizer
      第6回合成生物工学シンポジウム
    • Place of Presentation
      神戸大学(兵庫県神戸市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 密度勾配遠心法を利用した油脂酵母Lipomyces starkeyiの油脂高蓄積変異株の取得2015

    • Author(s)
      海老名沙也佳、阿部史歩、志田洋介、小笠原渉、山崎晴丈、高久洋暁
    • Organizer
      日本生物工学会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂酵母Lipomyces starkeyiにおける油脂低蓄積変異株の油脂合成・分解関連遺伝子の発現解析2015

    • Author(s)
      小黒雅智、山崎晴丈、志田洋介、小笠原渉、高久洋暁
    • Organizer
      日本生物工学会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂酵母Lipomyces starkeyiにおける油脂高生産培養条件の検討2015

    • Author(s)
      小黒芳史、澤井隆明、山崎晴丈、志田洋介、小笠原渉、高久洋暁
    • Organizer
      日本生物工学会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 油脂高蓄積株、油脂高蓄積株を製造する方法、油脂高蓄積株を用いて油脂を製造する方法、及び、油脂高蓄積株の抽出物2018

    • Inventor(s)
      高久洋暁、荒木秀雄、山崎晴丈
    • Industrial Property Rights Holder
      新潟薬科大学、不二製油グループ本社株式会社
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-034748
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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