Elucidation of mechanism for lipid production in oleaginous yeast
| Project/Area Number |
15K07372
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
Takaku Hiroaki 新潟薬科大学, 応用生命科学部, 教授 (70350717)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 油脂酵母 / Lipomyces / 油脂蓄積変異株 / 遺伝子発現 / 遺伝子組換え / トリアシルグリセロール / 脂肪酸合成 / 油脂高蓄積変異株 / アセチルCoA / トリアシルグリセロール分解酵素 / ベータ酸化 |
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| Outline of Final Research Achievements |
Biodiesel, one of next generation fuels, is mainly produced by transesterification of edible vegetable oils. The usage of edible oils as a biodiesel feedstock is often controversial because of the competition with food production. Therefore, we noted the oleaginous yeast Lipomyces starkeyi, which can convert non-edible biomass-derived carbohydrates to lipid. L. starkeyi is an excellent lipid producer that is able to accumulate up to 70% of its biomass as triacylglycerols. However, there are issues of lipid productivity for commercial applications.
In this study, we obtained of L. starkeyi mutants accumulating a high level of lipid. We extracted genes related to lipid biosynthesis by comparing gene expression profiles in the wild-type and mutants. The high-level expression of those extracted genes in the wild -type showed the high-level lipid production.
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Report
(4 results)
Research Products
(21 results)
