Pioneer research of transcription network of stringent control in Bacillus subtilis and its application
| Project/Area Number |
15K07374
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokai University (2017)
Fukuyama University (2015-2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
広岡 和丈 福山大学, 生命工学部, 准教授 (20389068)
小倉 光雄 東海大学, 海洋研究所, 教授 (80204163)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 枯草菌 / 胞子形成 / バイオフィルム / 緊縮応答 / 緊縮転写制御 / 発現制御 / ゲノム / プリン代謝制御 / 緊縮制御 / 転写抑制 / 正の緊縮転写制御 / kinB / SinR / 転写開始 / 転写制御 / 代謝制御 / 転写ネットワーク |
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| Outline of Final Research Achievements |
Transcription initiation of sporulation trigger genes of Bacillus subtilis (kinB and kinA) from adenine (+1) is activated upon stringent response, that is, under positive stringent transcription control. kinB transcription is repressed by a regulatory factor SinR for biofilm formation. Moreover, SinR was found to be involved in positive stringent transcription controlof kinB. The lacZ-fusion and EMSA analyses revealed a SinR-binding site (-61/-57) for SinR repression of kinB and another SinR-binding site (-29/-8) for positive strigent transcription control of kinB. Furthermore, SinR was involved not only in positive stringent transcription control of kinB but also in that of kinA, ilvB, and pycA.
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Report
(4 results)
Research Products
(7 results)
