| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Yeast has nitrogen catabolite repression (NCR) by which unnecessary production of enzymes/transporters for utilization of non-preferred nitrogen sources is suppressed when a preferred nitrogen source such as glutamate (Glu) is available. NCR prevents growth of auxotrophic mutants that require particular amino acids (poor nitrogen sources) for their growth. Amino acid auxotrophic mutants of fission yeast were unable to grow on minimal medium containing Glu even when adequate amounts of required amino acids were supplied. However, growth of the mutant cells was recovered near the prototrophic strain, suggesting that some substances secreted from the growing cells allowed the mutants to switch their nitrogen source preference. We identified two fatty acid molecules secreted from fission yeast that abrogate NCR and allow the cells to uptake poor nitrogen sources even in the presence of preferred ones. We also showed that the Agp3 amino acid transporter was involved in the adaptive growth.
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