Analysis of NAD metabolic pathway in Mycobacterium tuberculosis and elucidation of its compensatory pathway
| Project/Area Number |
15K07377
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
MORI Shigetarou 国立感染症研究所, 細菌第二部, 室長 (60425676)
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 結核菌 / タンパク質 / 酵素 / ドッキングシミュレーション / NAD生合成 / タンパク質発現 / 立体構造解析 / NAD合成経路 |
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| Outline of Final Research Achievements |
In this research project, we mainly analyzed the functions and structures of mycobacterial enzymes involved in the NAD biosynthetic pathway. The optimal conditions for expressing Rv0212c, Rv1901c, Rv3199c, and Rv3393 of Mycobacterium tuberculosis in Escherichia coli were determined. We found that Rv3393 shows nucleoside hydrolysis activity. In addition, we showed that the structure of the substrate-binding site of phosphoribosyl diphosphate synthase from M. smegmatis differs from that of human phosphoribosyl diphosphate synthase. Furthermore, the enzymatic properties of Rv3257c and Rv3308 from M. tuberculosis and the three-dimensional structure of the nucleotide phosphorylase from M. avium were determined in detail.
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Report
(4 results)
Research Products
(6 results)
