| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In plants, physiologically active compounds are inactivated by glycosylation and activated by hydrolysis of glycosides. In this study, rice beta-glucosidase TAGG2, which has high activity towards both tuberonic acid (TA) beta-glucoside and salicylic acid beta-glucoside (SAG), was biochemically and physiologically characterized. Important amino acid residues involved in recognition of SAG were determined through site-directed mutagenesis, and localization of TAGG2 to apoplast was confirmed using transgenic rice expressing a fusion protein of GFP and TAGG2. In transgenic rice expressing TAGG2, over-accumulation of TA was observed but SA level was not changed significantly. Blast infection analysis suggested overexpression of the TAGG2 gene negatively regulated the resistance against pathogens. Homologous enzymes in Arabidopsis had high activity towards oligosaccharide substrates such as laminarioligosaccharide rather than SAG.
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