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Molecular analysis and application trial of a novel enzyme for efficient production of valuable compounds from sesame oil cake

Research Project

Project/Area Number 15K07380
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionKanazawa University

Principal Investigator

Yamashita Satoshi  金沢大学, 物質化学系, 准教授 (70361186)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsセサミノール / 脂溶性有用物質
Outline of Final Research Achievements

This research focuses on the trials for efficient production of sesaminol, a sesame lignan with prominent health-giving effects on human such as potent antioxidant activity. Although sesame oil contains a small amount of sesaminol, sesame seed contains some conjugated forms of sesaminol such as sesaminoll triglucoside (STG) which is highly resistant to hydrolytic actions of beta-glucosidases. We previously isolated a beta-glucosidase which was capable of hydrolyzing STG to produce sesaminol, and named the enzyme as PSTG. However, the molecular mechanism of PSTG is still unknown and its possibility of application for industrial production of sesaminol needs to be examined. Here, we succeeded a consecutive reaction from STG to sesaminol in vitro. To obtain insights into the mechanism of PSTG, we crystallized PSTG and successfully obtained good crystals. Moreover, we identified the operon containing PSTG gene and a novel PSTG-like gene which encoded a more efficient enzyme than PSTG.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Identification and characterization of a novel bacterial β-glucosidase that is highly specific for the β-1,2-glucosidic linkage of sesaminol triglucoside2018

    • Author(s)
      Sakurai A., Yamashita S., Nakayama T. et al
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 00 Issue: 9 Pages: 00-00

    • DOI

      10.1080/09168451.2018.1476123

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] セサミノール配糖体の新規分解酵素の単離およびキャラクタリゼーション2017

    • Author(s)
      Yamashita S., Nakayama T., et al
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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