| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Ribonuclease H (RNase H) is an enzyme that specifically degrades RNA from RNA/DNA hybrids. RNase H has been classified into two major families, type 1 and type 2. Retroviral reverse transcriptase (RT) has DNA polymerase and RNase H activities. In this study, we aimed to elucidate the RNase H and engaged in the following four subjects. (i) The RNase H domain of MMLV RT was prepared and characterized. (ii) The effects of neutral salts and pH on the activity and stability of human RNase H2 were analyzed. (iii) The GPMA method using mouse NIH3T3 cell line instead of E. coli was established. (iv) The RNase H2 knock-out NIH3T3 cell line was established and characterized.
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