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Biochemical analysis of the generation of high density lipoprotein(HDL) by ABC proteins

Research Project

Project/Area Number 15K07390
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

Kimura Yasuhisa  京都大学, 農学研究科, 助教 (10415143)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsABCタンパク質 / HDL / コレステロール / 脂質輸送 / トランスポーター / ABCA1 / 高密度リポタンパク質 / 善玉コレステロール / ABCG1
Outline of Final Research Achievements

High density lipoprotein (HDL) is the sole pathway to remove the excess cholesterol from peripheral tissues. The amounts/production of HDL reversely correlated to atherosclerosis, HDL is also known as “good cholesterol”. In this study, we performed biochemical analysis of ATP-dependent lipid transporter, ABCA1. We performed the biochemical analysis for ABCA1 and apoA-I interaction. We also demonstrated that ABCA1 temporarily sequesters phospholipids and cholesterol in large extracellular domain and generates HDL by using these lipid. We also purified several lipid transporting ABC transporters and analyzed their substrate specificity.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (22 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Journal Article] Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation.2018

    • Author(s)
      Masato Ishigami, Fumihiko Ogasawara, Kohjiro Nagao, Hidehiko Hashimoto, Yasuhisa Kimura, Noriyuki Kioka and Kazumitsu Ueda
    • Journal Title

      Sci Rep.

      Volume: 8 Issue: 1 Pages: 6170-6170

    • DOI

      10.1038/s41598-018-24428-6

    • NAID

      120006502578

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vinculin Association with Actin Cytoskeleton is Necessary for Stiffness-dependent Regulation of Vinculin Behavior.2017

    • Author(s)
      Tomohiro Omachi, Takafumi Ichikawa, Yasuhisa Kimura, Kazumitsu Ueda, and Noriyuki Kioka.
    • Journal Title

      PLos One

      Volume: 12 Issue: 4 Pages: e0175324-e0175324

    • DOI

      10.1371/journal.pone.0175324

    • NAID

      120006309292

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vinculin promotes nuclear localization of TAZ to inhibit ECM stiffness-dependent differentiation into adipocytes2017

    • Author(s)
      Mito Kuroda, Hiroki Wada, Yasuhisa Kimura, Kazumitsu Ueda and Noriyuki Kioka
    • Journal Title

      Journal of Cell Science

      Volume: 130 Issue: 5 Pages: 989-1002

    • DOI

      10.1242/jcs.194779

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Functional analysis of transmembrane helix 4 of human ABCB42018

    • Author(s)
      〇Shun Ogawa, Kento Suenaga, Noriyuki Kioka, Yasuhisa Kimura, Kazumitsu Ueda
    • Organizer
      7th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Substrate entry mechanism of human MDR12018

    • Author(s)
      Yasuhisa Kimura, Shuichi Inoue, Noriyuki Kioka, Kazumitsu Ueda
    • Organizer
      7th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Related Report
      2017 Annual Research Report
  • [Presentation] リン脂質輸送体ABCB4の 基質取り込みゲートの解析2018

    • Author(s)
      小川峻、末永健人、木岡紀幸、木村泰久、植田和光
    • Organizer
      日本農芸化学会 2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光反応性ホスファチジルコリンを用いた脂質抱合型apoA-Iの標識手法の確立2018

    • Author(s)
      藤本和希、木村泰久、安部真人、三芳秀人、植田和光
    • Organizer
      日本農芸化学会 2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コレステロール認識配列CRACは、ヒトABCG4のコレステロール輸送において重要である2017

    • Author(s)
      見月俊吾、木村泰久、木岡紀幸、松尾道憲、植田和光
    • Organizer
      2017年 日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒトABCB6によるポルフィリン認識機構の解析2017

    • Author(s)
      西澤奈美、大貫元、木村泰久、木岡紀幸、植田和光
    • Organizer
      2017年 日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of thermostabilizing mechanism of multidrug transporter2017

    • Author(s)
      Yilun WANG, Shuichi INOUE, Noriyuki KIOKA, Yasuhisa KIMURA, Kazumitsu UEDA,
    • Organizer
      2017年 日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒトABCB4の基質取り込み経路の検討2017

    • Author(s)
      小川峻、末永健人、木岡紀幸、木村泰久、植田和光
    • Organizer
      "日本農芸化学会 関西・中四国・ 西日本支部 2017年度 合同大阪大会"
    • Related Report
      2017 Annual Research Report
  • [Presentation] 定量的アプローチによるABCA1とapoA-Iによる脂質排出メカニズムの解明2017

    • Author(s)
      三好里奈、小笠原史彦、石神正登、永田紅、木村泰久、木岡紀幸、植田和光
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポルフィリンのカルボキシル基はヒトABCB6によるポルフィリン認識に重要である2017

    • Author(s)
      西澤奈美、木村泰久、大貫元、木岡紀幸、植田和光
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒトABCB4の第4膜貫通ヘリックスの機能解析2017

    • Author(s)
      小川峻、末永健人、木岡紀幸、木村泰久、植田和光
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヌクレオチド依存的なKATP チャネル複合体の構造変化2016

    • Author(s)
      木村泰久
    • Organizer
      第1 回 イオンチャネル研究会
    • Place of Presentation
      福岡
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ヒト培養細胞を用いた哺乳類膜タンパク質複合体の発現・精製2016

    • Author(s)
      木村泰久
    • Organizer
      第16回 日本蛋白質科学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ヒトABCG4に存在するコレステロール認識配列の機能解析2016

    • Author(s)
      見月俊吾、木村泰久、木岡紀幸、松尾道憲、植田和光
    • Organizer
      2016年(平成28年度)日本農芸化学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 原子間力顕微鏡(AFM)観察に向けたABCタンパク質サンプルの最適化2016

    • Author(s)
      木村泰久、菅野亮、松尾道憲、加藤博章、光岡薫、植田和光
    • Organizer
      ヌクレオチド依存的なATP感受性カリウムチャネルの構造変化
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Reconstitution of human ABCA1 in nanodisc for the functional analysis2016

    • Author(s)
      川野邊峻哲、木村泰久、木岡紀幸、植田和光
    • Organizer
      6th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Place of Presentation
      Innsbruck, Austria
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of the CRAC domain in TM6 of human ABCG4.2016

    • Author(s)
      見月俊吾、木村泰久、木岡紀幸、松尾道憲、植田和光
    • Organizer
      6th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Place of Presentation
      Innsbruck, Austria
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of functional role of TMD0 of human ABCB62016

    • Author(s)
      西澤奈美、大貫元、木村泰久、木岡紀幸、植田和光
    • Organizer
      6th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Place of Presentation
      Innsbruck, Austria
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Nucleotide induces conformational change in the channel subunit of KATP channel2016

    • Author(s)
      木村泰久、菅野亮、松尾道憲、加藤博章、光岡薫、植田和光
    • Organizer
      6th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
    • Place of Presentation
      Innsbruck, Austria
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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