Biochemical analysis of the generation of high density lipoprotein(HDL) by ABC proteins
| Project/Area Number |
15K07390
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ABCタンパク質 / HDL / コレステロール / 脂質輸送 / トランスポーター / ABCA1 / 高密度リポタンパク質 / 善玉コレステロール / ABCG1 |
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| Outline of Final Research Achievements |
High density lipoprotein (HDL) is the sole pathway to remove the excess cholesterol from peripheral tissues. The amounts/production of HDL reversely correlated to atherosclerosis, HDL is also known as “good cholesterol”. In this study, we performed biochemical analysis of ATP-dependent lipid transporter, ABCA1. We performed the biochemical analysis for ABCA1 and apoA-I interaction. We also demonstrated that ABCA1 temporarily sequesters phospholipids and cholesterol in large extracellular domain and generates HDL by using these lipid. We also purified several lipid transporting ABC transporters and analyzed their substrate specificity.
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Report
(4 results)
Research Products
(22 results)
