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Molecular mechanism of abundant biosynthesis and accumulation of ascorbic acid in tropical plant acerola.

Research Project

Project/Area Number 15K07394
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionHiroshima University

Principal Investigator

Esaka Muneharu  広島大学, 生物圏科学研究科, 教授 (70151975)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsアスコルビン酸 / アセロラ / 遺伝子発現 / 遺伝子組換え / ビタミンC / プロモーター / 転写調節 / アスコルビン酸ペルオキシダーゼ / ガラクツロン酸レダクターゼ / プロモーター解析 / 酵素 / 転写因子
Outline of Final Research Achievements

Ascorbic acid is an antioxidant, which is abundantly contained within the plant cell, and plays important roles in plants. Plants have some pathways for ascorbic acid biosynthesis. The mechanism for biosynthesis regulation of plant ascorbic acid attracts so much attention.
Acerola (Malpighia glabra L.) is one of the richest known natural sources of ascorbic acid (vitamin C). Thus, the gene expression of ascorbic acid-biosynthetic enzymes is markedly high in acerola. Molecular mechanism of the high expression of the ascorbic acid biosynthetic enzyme gene became clear partially. Acerola genes for ascorbic acid-biosynthetic enzymes were introduced and over-expressed to generate transgenic plants containing a large amount of ascorbic acid. Finally, this research will lead to generation of stress-resistant transgenic plants with high ascorbic acid contents.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Two G-box like elements essential to high gene expression of SlAKR4B in tomato leaves.2018

    • Author(s)
      Suekawa M, Fujikawa Y and Esaka M
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 3 Pages: 425-432

    • DOI

      10.1080/09168451.2018.1429887

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel regulatory element responsible for high transcriptional expression of acerola GDP-d-mannose pyrophosphorylase gene.2017

    • Author(s)
      Kondo T., Fujikawa Y., Esaka M.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 印刷中 Issue: 6 Pages: 1194-1197

    • DOI

      10.1080/09168451.2017.1285690

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses.2016

    • Author(s)
      Suekawa M., Fujikawa Y., Inada S., Murano A., Esaka M.
    • Journal Title

      J. Plant Physiol.

      Volume: 200 Pages: 35-44

    • DOI

      10.1016/j.jplph.2016.05.015

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cloning and gene expression analysis of ascorbic acid biosynthesis enzymes in Moringa oleifera.2015

    • Author(s)
      Kondo, T., Fujikawa, Y., Ueda, A., Nagaoka, T., Saneoka, H., Martinez, M, Calcano, M., Martich, J.D.H., Esaka, M.
    • Journal Title

      African Journal of Agricultural Research

      Volume: 10 Pages: 2274-2285

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] トマトにおけるアスコルビン酸生合成酵素ガラクツロン酸レダクターゼの 遺伝子発現機構2018

    • Author(s)
      末川 麻里奈,藤川 愉吉,江坂 宗春
    • Organizer
      第32回バイオテクノロジー研究成果発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トマトにおけるアルドケト還元酵素の遺伝子発現機構と機能解析2017

    • Author(s)
      末川 麻里奈、藤川 愉吉、江坂 宗春
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] トマトにおけるアルドケト還元酵素の遺伝子発現機構に関する研究2017

    • Author(s)
      末川麻里奈,藤川愉吉,江坂宗春
    • Organizer
      日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The analysis of gene expression mechanism and physiological function of aldo-keto reductase in tomato.2017

    • Author(s)
      Suekawa M., Fujikawa Y., Esaka M.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アセロラのアスコルビン酸生合成酵素GDP-D-mannose pyrophosphorylaseの転写調節因子の探索2016

    • Author(s)
      近藤 隆之、藤川 愉吉、江坂 宗春
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
  • [Presentation] トマトのアスコルビン酸生合成におけるガラクツロン酸レダクターゼの発現機構と生理機能2016

    • Author(s)
      末川 麻里奈、藤川 愉吉、江坂 宗春
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
  • [Presentation] アセロラのアスコルビン酸大量集積機構の解明2016

    • Author(s)
      井上 明香里、近藤 隆之、藤川 愉吉、江坂 宗春
    • Organizer
      第30回バイオテクノロジー研究成果発表会
    • Place of Presentation
      広島市まちづくり市民交流プラザ
    • Year and Date
      2016-02-04
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] Regulation of ascorbic acid biosynthesis in plants., In “Ascorbic acid in plant growth, development and stress tolerance”.2017

    • Author(s)
      Suekawa M., Kondo T., Fujikawa Y., Esaka M.
    • Total Pages
      514
    • Publisher
      Springer International Publishing
    • ISBN
      9783319740560
    • Related Report
      2017 Annual Research Report
  • [Book] Physiological role of ascorbic acid recycling enzymes in plants., In “Ascorbic acid in plant growth, development and stress tolerance”.2017

    • Author(s)
      Suekawa M., Fujikawa Y., Esaka M.
    • Total Pages
      514
    • Publisher
      Springer International Publishing
    • ISBN
      9783319740560
    • Related Report
      2017 Annual Research Report
  • [Remarks] 江坂宗春教授/教授に聞く/広島大学

    • URL

      http://home.hiroshima-u.ac.jp/gsbstop/interview/ja/esaka.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 江坂 宗春 (大学院生物圏科学研究科)

    • URL

      http://seeds.office.hiroshima-u.ac.jp/profile/ja.a3d4f760e9b22e0c520e17560c007669.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究者総覧 江坂宗春

    • URL

      http://seeds.office.hiroshima-u.ac.jp/profile/ja.a3d4f760e9b22e0c520e17560c007669.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 酵素化学 江坂

    • URL

      http://home.hiroshima-u.ac.jp/enzyme/esaka.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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