Elucidation of the parasporin-1 receptor and effect of the anti-parasporin-1 receptor antibody to human cell lines
| Project/Area Number |
15K07403
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Fukuoka Industrial Technology Center |
|---|
Principal Investigator |
Okumura Shiro 福岡県工業技術センター, その他部局等, 専門研究員 (40399671)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KATAYAMA HIDEKI 福岡県工業技術センター, 生物食品研究所, 専門研究員 (90416496)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | パラスポリン / 受容体 / 細胞傷害活性 / がん / 細胞傷害性 / ヒト培養細胞 / 細胞応答 / ベクリン1 |
|---|
| Outline of Final Research Achievements |
The purpose of this study is to investigate the receptor of parasporin-1 (PS1) and investigate in detail the phenomenon that antibody against beclin-1 which is a potent receptor candidate induces apoptosis of cancer cells like PS1. For the search of receptors for PS1, we have searched with a photocrosslinking probe immobilized with PS1 and carried out analysis by western blotting, however, the reproducibility of the analysis result was not good. But we found that there is a correlation with the calcium concentration and the result of analysis. In addition, we produced recombinant beclin-1 by E. coli, and the monoclonal antibody was prepared from the protein. The cytotoxic activity against PS1-susceptible cell lines and the inhibitory ability against PS1 of the affinity purified antibody were measured, but neither activity was observed.
|
Report
(4 results)
Research Products
(5 results)
