| Project/Area Number |
15K07403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Fukuoka Industrial Technology Center |
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Principal Investigator |
Okumura Shiro 福岡県工業技術センター, その他部局等, 専門研究員 (40399671)
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| Co-Investigator(Renkei-kenkyūsha) |
KATAYAMA HIDEKI 福岡県工業技術センター, 生物食品研究所, 専門研究員 (90416496)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | パラスポリン / 受容体 / 細胞傷害活性 / がん / 細胞傷害性 / ヒト培養細胞 / 細胞応答 / ベクリン1 |
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| Outline of Final Research Achievements |
The purpose of this study is to investigate the receptor of parasporin-1 (PS1) and investigate in detail the phenomenon that antibody against beclin-1 which is a potent receptor candidate induces apoptosis of cancer cells like PS1. For the search of receptors for PS1, we have searched with a photocrosslinking probe immobilized with PS1 and carried out analysis by western blotting, however, the reproducibility of the analysis result was not good. But we found that there is a correlation with the calcium concentration and the result of analysis. In addition, we produced recombinant beclin-1 by E. coli, and the monoclonal antibody was prepared from the protein. The cytotoxic activity against PS1-susceptible cell lines and the inhibitory ability against PS1 of the affinity purified antibody were measured, but neither activity was observed.
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