Elucidation of the action mechanism of the insect-selective peptide toxin LaIT1 isolated from the Liocheles australasiae scorpion venom

• Project/Area Number: 15K07412
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Kyoto University
• Principal Investigator: Miyashita Masahiro 京都大学, 農学研究科, 准教授 (80324664)
• Co-Investigator(Renkei-kenkyūsha): MATSUDA Kazuhiko 近畿大学, 農学部, 教授 (00199796)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ペプチド / 生物毒 / 神経毒 / 光親和性標識 / 殺虫性ペプチド / サソリ毒 / 電気泳動 / 質量分析 / ビオチン / 受容体タンパク質 / scorpion toxin / receptor binding / iodination / insect toxicity / peptide

Outline of Final Research Achievements

LaIT1 is an insect-selective peptide toxin isolated from the Liocheles australasiae scorpion venom. In this study, a photoaffinity-labeled LaIT1 analog was synthesized to identify proteins that specifically bind LaIT1 in the central nervous system of the silkworm larvae. The candidate proteins have not been reported as an action target of scorpion toxins, suggesting that LaIT1 has an unknown action mechanism.

Research Products

• [Journal Article] Complete de novo sequencing of antimicrobial peptides in the venom of the scorpion Isometrus maculatus (2017)
  • Author(s): Miyashita, M. Kitanaka, A. Yakio, M. Yamazaki, Y. Nakagawa, Y. Miyagawa, H.
  • Journal Title: Toxicon Volume: 139 Pages: 1-12
  • DOI: 10.1016/j.toxicon.2017.09.010
  • Peer Reviewed
• [Journal Article] Fundamental and Practical Aspects of <i>de novo</i> Peptide Sequencing (2017)
  • Author(s): 宮下正弘
  • Journal Title: Journal of the Mass Spectrometry Society of Japan Volume: 65 Issue: 6 Pages: 231-238
  • DOI: 10.5702/massspec.17-89
  • NAID: 130006250293
  • ISSN: 1340-8097, 1880-4225, 1884-3271
  • Open Access
• [Journal Article] A Facile Method for Preferential Modification of the N-Terminal Amino Group of Peptides Using Triazine-Based Coupling Reagents (2017)
  • Author(s): Kitanaka, A. Juichi, H. Nihashi, Y. Miyashita, M. Miyagawa, H.
  • Journal Title: Mass Spectrometry Volume: 6 Issue: 1 Pages: A0059-A0059
  • DOI: 10.5702/massspectrometry.A0059
  • NAID: 130005906777
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed / Open Access
• [Journal Article] Isolation, structural identification and biological characterization of two conopeptides from the Conus pennaceus venom (2017)
  • Author(s): Abdel-Wahab, M. Miyashita, M. Ota, Y. Juichi, H. Okabe, R. Sarhan, M. Fouda, M. Abdel-Rahman, M. Saber, S. Nakagawa, Y.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 81 Issue: 11 Pages: 1879-1882
  • DOI: 10.1080/09168451.2017.1364966
  • Peer Reviewed / Int'l Joint Research
• [Presentation] ヤエヤマサソリの毒液に含まれる抗菌性ペプチドの探索と同定 (2017)
  • Author(s): 岡部諒太、北中淳史、内山博允、須恵雅之、宮下正弘、中川好秋、宮川恒
  • Organizer: 日本農芸化学会2017年度大会
• [Presentation] Chemical modification-assisted de novo sequencing of antimicrobial peptides in the scorpion venom (2017)
  • Author(s): M. Miyashita, A. Kitanaka, M. Yakio, Y. Yamazaki, Y. Nakagawa, H. Miyagawa
  • Organizer: 19th World Congress of the International Society on Toxinology
  • Int'l Joint Research
• [Presentation] De novoシーケンス解析とトランスクリプトーム解析の組み合わせによるヤエヤマサソリ毒液に含まれる抗菌性ペプチドの構造決定 (2017)
  • Author(s): 宮下正弘、北中淳史、岡部諒太、内山博允、須恵雅之、中川好秋、宮川恒
  • Organizer: 第65回質量分析総合討論会
• [Presentation] Identification of antimicrobial peptides from the Liocheles australasiae scorpion venom using de novo sequencing and transcriptomic analyses (2017)
  • Author(s): R. Okabe, A. Kitanaka, H. Uchiyama, M. Sue, M. Miyashita, Y. Nakagawa, H. Miyagawa
  • Organizer: 第54回ペプチド討論会